ASSESSMENT OF PLACENTAL OXIDATIVE STRESS PARAMETERS IN PREECLAMPTIC AND NORMAL PREGNANT WOMEN
Objective: Oxidative stress occurs when cellular levels of reactive oxygen species exceed antioxidant capabilities and has been implicated in the
pathogenesis of pre-eclampsia. In this study, we examined the tissue levels of endogenous antioxidant enzymes or proteins (superoxide dismutase
[SOD], glutathione peroxidise, reduced glutathione, catalase, and thioredoxin) and the levels of lipid peroxides, protein carbonyls, hydrogen peroxide,
and nitrosative biomarkers in the placental samples from normal and pre-eclamptic pregnancies.
Results: Pre-eclamptic tissue homogenates demonstrated significantly increased levels of lipid peroxidation (21.61Â±0.18 vs. 5.695Â±0.46) and
a trended increase in protein carbonyls (245.95Â±4.05 vs. 203.48Â±3.65) concentration when compared to controls. The levels and activities of the
antioxidant proteins; SOD (365.2Â±2.915 vs. 205.6Â±3.76), thioredoxin (100.64Â±3.38 vs. 80.89Â±3.37), glutathione peroxidase (340.88Â±6.16 vs.
164.46Â±3.03), catalase (5.26Â±0.02 vs. 4.62Â±0.11), and reduced glutathione (46.99Â±0.508 vs. 28.19Â±0.178) were all found to be significantly reduced
when comparing pre-eclamptic placental tissue homogenates to gestational age matched control placentae from non pre-eclamptic pregnancies.
Conclusion: The results of this study demonstrate a decreased enzymatic antioxidant capacity and increased oxidation in placental tissue from preeclamptic
the pathogenesis of this complex
Keywords: Oxidative stress, Reactive oxygen species, Superoxide dismutase, Glutathione peroxidise, Reduced glutathione, Catalase, Thioredoxin,
Lipid peroxides and protein carbonyls.
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