ASSESSMENT OF PLACENTAL OXIDATIVE STRESS PARAMETERS IN PREECLAMPTIC AND NORMAL PREGNANT WOMEN

  • Kavitha V
  • SRINIVAS B kakatiya university
  • Praveen Kumar Mvln
  • Shashikanth P

Abstract

Objective: Oxidative stress occurs when cellular levels of reactive oxygen species exceed antioxidant capabilities and has been implicated in the
pathogenesis of pre-eclampsia. In this study, we examined the tissue levels of endogenous antioxidant enzymes or proteins (superoxide dismutase
[SOD], glutathione peroxidise, reduced glutathione, catalase, and thioredoxin) and the levels of lipid peroxides, protein carbonyls, hydrogen peroxide,
and nitrosative biomarkers in the placental samples from normal and pre-eclamptic pregnancies.
Results: Pre-eclamptic tissue homogenates demonstrated significantly increased levels of lipid peroxidation (21.61±0.18 vs. 5.695±0.46) and
a trended increase in protein carbonyls (245.95±4.05 vs. 203.48±3.65) concentration when compared to controls. The levels and activities of the
antioxidant proteins; SOD (365.2±2.915 vs. 205.6±3.76), thioredoxin (100.64±3.38 vs. 80.89±3.37), glutathione peroxidase (340.88±6.16 vs.
164.46±3.03), catalase (5.26±0.02 vs. 4.62±0.11), and reduced glutathione (46.99±0.508 vs. 28.19±0.178) were all found to be significantly reduced
when comparing pre-eclamptic placental tissue homogenates to gestational age matched control placentae from non pre-eclamptic pregnancies.
Conclusion: The results of this study demonstrate a decreased enzymatic antioxidant capacity and increased oxidation in placental tissue from preeclamptic
women,
which
may
contribute
to
the pathogenesis of this complex
disorder.
Keywords: Oxidative stress, Reactive oxygen species, Superoxide dismutase, Glutathione peroxidise, Reduced glutathione, Catalase, Thioredoxin,
Lipid peroxides and protein carbonyls.

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How to Cite
V, K., S. B, P. K. Mvln, and S. P. “ASSESSMENT OF PLACENTAL OXIDATIVE STRESS PARAMETERS IN PREECLAMPTIC AND NORMAL PREGNANT WOMEN”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 1, Jan. 2016, pp. 105-9, https://innovareacademics.in/journals/index.php/ajpcr/article/view/8948.
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