PLANT MEDIATED SYNTHESIS OF SILVER NANOPARTICLES WITH DIVERSE APPLICATIONS
Objective: Green synthesis of multifunctional silver nanoparticles (SNP) using leaf extracts of Simarauba glauca and Artocarpus altilis, a good alternative to the electrochemical methods were exploited in this study for potential use as biomedical agent in medical field.
Methods: Synthesis of silver nanoparticles using leaf extracts of Simarauba glauca and Artocarpus altilis was carried out. The SNPs were characterized by UV-visible spectrophotometry, Scanning electron microscopy (SEM) and Energy Dispersion spectral analysis (EDS) and evaluated for their multifunctionality.
The SNP were synthesised from the leaf extracts of Simarauba glauca and Artocarpus altilis using silver nitrate with the reaction time of two min and 10 min respectively. UV-visible absorption scan revealed a characteristic peak at 420nm indicative of the surface plasmon resonance for SNP. The synthesized silver nanoparticles were of size ranges from 33-50nm and 37.9 nm synthesised from the leaf extracts of Simarauba glauca and Artocarpus altilis respectively. Further the synthesized silver nanoparticles have shown excellent antibacterial activity against test pathogens, applicability in sewage water treatment and biofilm degradation and having perfect haemcompatibility.
Conclusion: The biologically synthesized silver nanoparticles with these functionalities has transformed it to a multifunctional biomedical agent for potential use in medical field.
2. Ingale AG, Chaudhari AN. Biogenic synthesis of nanoparticles and potential applications: An eco- friendly approach. J Nanomed Nanotechnol 2013; 4 (2): 1-7.
3. Karnani RL, Chowdhary A. Biosynthesis of silver nanoparticle by eco-friendly method. Indian J Nano Sci 2013; l (2):25-31.
4. Dhanasekaran S, Karunakaran S, Amutha R, Suruthipriyadharshin, S, Jayalakshmi K. Biosynthesis of silver nanoparticles using Acorus calamus and its antibacteral activity. International Journal of Nanomaterials and Biostructures 2014; 4(1): 16-20.
5. Singh K, Panghal M, Kadyan S, Chaudary U, Yadav PJ. Antibacterial activity of synthesized silver nanoparticles from Tinospora cordifolia against multi drug resistant strains of Pseudomonas aeruginosa isolated from burn patients. J Nanomed Nanotechnol 2014; 5 (192):2157-7439.
6. Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 2007; 42:321â€“324.
7. Sambanthamoorthy K, Feng X, Patel R, Patel S, Paranavitana C. Antimicrobial and antibiofilm potential of biosurfactants isolated from Lactobacilli against multi-drug-resistant pathogens. BMC Microbiol 2014; 14: 1-9.
8. Nanda A, Saravanan M. Biosynthesis of silver nanoparticles from Staphylococcus aureusand its antimicrobial activity against MRSA and MRSE. Nanomedicine 2009; 5: 452-456.
9. Laloy J, Minet V, Alpan L, Mullier F, Beken S, Toussaint O, Lucas S, DognÃ© JM. Impact of silver nanoparticles on haemolysis, platelet function and coagulation. Nanobiomedicine 2014; 1(4): 1-9.
10. Gurunathan S, Lee KJ, Kalishwaralal K, Sheikpranbabu S, Vaidyanathan R, Eom SH. Antiangiogenic properties of silver nanoparticles. Biomaterials 2009; 30:6341â€“6350.
11. Seshadri S, Prakash A, Kowshik M. Biosynthesis of silver nanoparticles by marine bacterium Idiomarina sp. PR58-8. Bull Mater Sci 2012; 35(7):1201â€“1205.
12. Moideen S, Lakshmi P. Green synthesis of silver nanoparticles using Luffa acutangula roxb. Var. Amara. Lin and its antibacterial activity. International Journal of pharma and bio sciences 2014; 5(4):1051â€“1061.
13. Awwad M, NidÃ¡ S, Amany A. Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity. International Journal of Industrial Chemistry 2013; 4(29):2-6.
14. Sulaiman GM, Mohammed WH, Marzoog TR, Al- Amiery AA, Kadhum AAH, Mohamad AB. Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract. Asian Pac J Trop Biomed 2013; 3(1): 58-63.
15. Ansari M, Khan M, Malik A, Azam A. Evaluation of antibacterial activity of silver nanoparticles against MSSA and MRSA on isolates from skin infections. Biology and Medicine 2011; 3(2): 141-146.
16. Kalishwaralal K, ManiKanth SB, Pandian SRK, Deepak V, Gurunathan S. Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis. Colloids and Surfaces B: Biointerfaces.2010; 79: 340â€“344.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.