A STUDY ABOUT ANTHELMINTIC EFFECT OF PUNICA GRAMATUM L BARK ON VETERINARY ENDOPARASITES


Nguyen Thanh Hai, Nguyen Van Thanh, Bui Thi Tho, Miyamoto Atsushi

Abstract


Objective: The present research was performed to evaluate the anthelmintic activity of Punica gramatum L plant bark on veterinary endoparasites. Method: We used 4 methods to extract the bark of Punica gramatum L plant, including using water, water and heat, water with previous soak in NaOH 5 % and water with previous soak in CH3COOH 5 %. Those solutions then were tested with animal endoparasites, including porcine ascarids, porcine cestodes, chicken ascarids and chicken trematodes at the concentrations of 20, 10, 5, 2.5 and 1.25 %. Their anthelmintic activity was evaluated through the lethal time by which the treatment of extracts induced the death of tested parasites. Result: bark of Punica gramatum L plant possesses anthelmintic efficacy with animal endoparasites. All of extracts at 5 % were able to kill 100% of experimental parasites within the tested time (360 minutes). Extraction with CH3COOH 5 % had the best effect, with the shortest necessary treatment time in all of tested concentrations with 4 experimental endoparasites. Conclusion: Punica gramatum L plant bark has in vitro anthelmintic on porcine ascarids, porcine cestodes, chicken ascarids and chicken trematodes, and follow-up researchs are necessary to assess the in vivo effect.

Key words:  Punica gramatum L, bark, extract, anthelmintic effect, endoparasites    

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About this article

Title

A STUDY ABOUT ANTHELMINTIC EFFECT OF PUNICA GRAMATUM L BARK ON VETERINARY ENDOPARASITES

Date

01-04-2014

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 7 Issue 2 (April-June) 2014 Page: 148-152

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Nguyen Thanh Hai

Nguyen Van Thanh

Bui Thi Tho

Miyamoto Atsushi


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