• PRABHJOT SINGH JASSAL Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Punjab – 1440111, Tamil Nadu, India.
  • MONIKA SHARMA Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Punjab – 1440111, Tamil Nadu, India.


Objective: The study was conducted for the evaluation of antioxidant, antibacterial, antihemolytic, and phytochemical activity of Ficus benjamina (FB), Ficus infectoria (FI), and Ficus krishnae (FK).

Methods: The antioxidant analysis of FB, FI, and FK was done by 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and nitric oxide (NO) scavenging assay. Evaluation was performed for antibacterial activity against Clostridium perfringens and Staphylococcus aureus by an agar well diffusion method. Hemolytic assay was performed with red blood cell suspension for evaluating antihemolytic activity. Standard protocols were used for phytochemical activity.

Results: In the results of DPPH antioxidant activity assay, it was found that DPPH inhibition was significantly increased with increasing amount of extract. For NO scavenging assay, ANOVA revealed that the inhibition of NO is not significantly affected with an increase in the amount of extract used. Results of antibacterial activity revealed that methanolic extract of FI shows a maximum zone of inhibition (30.5 mm) against S. aureus and aqueous extract of FK shows a maximum zone of inhibition (28 mm) against C. perfringens. The antihemolytic activity of FB, FI, and FK was performed by measuring percentage inhibition of plant extracts at different concentrations. FK has shown maximum percentage inhibition activity, i.e., 28.64 % at 60 μg/ml, whereas FB shows minimum inhibition activity, i.e. 2.7 % at 40 μg/ml. Flavonoid content was found to be 0.593 μg/ml, 0.783 μg/ml, and 1.023 μg/ml, whereas phenolic content was found to be 0.267 μg/ml, 0.298 μg/ml, and 0.355 μg/ml for FB, FI, and FK, respectively.

Conclusion: FB, FI, and FK extracts contain various phytochemicals which confirm that these plants can be used for therapeutic use and traditional medicine. The methanolic as well as aqueous extracts of the plants have shown the potential to kill the tested microorganism (C. perfringens and S. aureus) and hence can be used as an antibiotic and potential antibacterial.

Keywords: Antibacterial, Extracts, Phytochemicals, Antioxidant activity.

Author Biography

PRABHJOT SINGH JASSAL, Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Punjab – 1440111, Tamil Nadu, India.

Assistant Professor, Lovely Professional University


1. Ghani, A. Medicinal Plants of Bangladesh: Chemical Constituents and Uses. Dhaka: Asiatic Society of Bangladesh; 1998.
2. Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN, Ray C. Screening of Indian plants for biological activity: I. Indian J Exp Biol 1968;6:232-47.
3. Mousa O, Vuorela P, Kiviranta J, Törnquist K, Vuorela H, Hiltunen R. Bioactivity of certain Egyptian Ficus species. Plant Med 1992;58:632-3.
4. Elgimabi ME. Improvement of propagation by hardwood cuttings with or without using plastic tunnel in Hamelia patens. World J Agric Sci 2009;5:522-4.
5. Rzepka-Plevnes D, Kurek J. The Influence of Media Composition on the Proliferation and Morphology of Ficus benjamina plantlets. In: IV International Symposium on In Vitro Culture and Horticultural Breeding, ISHS Acta Horticulturae 560. 2000. p. 473-6.
6. Almahy HA, Rahmani M, Sukari MA, Ali AM. The chemical constituents of Ficus benjamina Linn. And their biological activities. Pertanika J Sci Technol 2003;11:73-81.
7. Sasi S, Anjum N, Tripathi YC. Ethnomedicinal, phytochemical and pharmacological aspects of Flacourtia jangomas: A review. Int J Pharm Pharm Sci 2018;10:9-15.
8. Singh D, Singh B, Goel RK. Traditional uses, phytochemistry and pharmacology of Ficus religiosa: A review. J Ethnopharmacol 2011;134:565-83.
9. da Rocha AB, Lopes RM, Schwartsmann G. Natural products in anticancer therapy. Curr Opin Pharmacol 2001;1:364-9.
10. Husain A, Virmani OP. Dictionary of Indian Medicinal Plants. Lucknow, India: Central Institute of Medicinal and Aromatic Plants; 1992.
11. Ambike SH, Rao MR. Studies on phytosterolin from the bark of Ficus religiosa. Znd J Pharm 1967;29:91
12. Swami KD, Bisht NP. Constituents of Ficus religiosa and Ficus infectoria and their biological activity. J Indian Chem Soc 1996;73:631.
13. Kumari M, Sharma A, Jagannadham MV. Decolorization of crude latex by activated charcoal, purification and physico-chemical characterization of religiosin, a milk-clotting serine protease from the latex of Ficus religiosa. J Agric Food Chem 2010;58:8027-34.
14. Biswas K. Observations on the systematic position of Ficus krishnae. Curr Sci 1934;3:424-7.
15. Shukla R, Gupta S, Gambhir JK, Prabhu KM, Murthy PS. Antioxidant effect of aqueous extract of the bark of Ficus bengalensis in hypercholesterolaemic rabbits. J Ethnopharmacol 2004;92:47-51.
16. Charde RM, Dhongade HJ, Charde MS, Kasture AV. Evaluation of antioxidant, wound healing and anti-inflammatory activity of ethanolic extract of leaves of Ficus religiosa. Int J Pharm Sci Res 2010;19:73-82.
17. Sandhu A, Bhardwaj N, Gupta R, Menon V. Antimicrobial activity and photochemical screening of Tinospora cordifolia and Euphorbia hirta. Int J Appl Biol Pharm Technol 2013;4:310-6.
18. Varier PS. Indian Medicinal Plants: A Compendium of 500 Species. Vol. 1. Kottakkal-India: Orient Longman Publishing House; 2002. p. 146.
19. Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I. Antioxidant principles from bauhinia t arapotensis. J Nat Prod 2001;64:892-5.
20. Taylor BS, Kim YM, Wang Q, Shapiro RA, Billiar TR, Geller DA, et al. Nitric oxide down-regulates hepatocyte-inducible nitric oxide synthase gene expression. Arch Surg 1997;132:1177-83.
21. Ugochukwu SC, Uche A, Ifeanyi O. Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennetia tripetala G. Baker. Asian J Plant Sci Res 2013;3:10-3.
22. Thephinlap C, Pangjit K, Suttajit M, Srichairatanakool S. Anti-oxidant properties and anti-hemolytic activity of Psidium guajava, Pandanous odorus and Rhinacanthus nasutus. J Med Plants Res 2013;7:1849-57.
23. Shamila IM, Jeeva S, Sheela DJ, Brindha JR, Lekshmi NC. Antimicrobial spectrum and phytochemical study of Ficus tsiela L. (Moreceae) Drug Invent Today 2012;4:337-9.
24. Tijjani AM, Abdurahaman IF, Buba WS, Mala IG, Akan CJ, Babakura MA. Chemical and proximate contents of methanolic leaf extract of Piliostigma thonningii Schum (Camel foot). J Chem Pharm Res 2012;4:2409-14.
25. Fouche G, Cragg GM, Pillay P, Kolesnikova N, Maharaj VJ, Senabe J, et al. In vitro anticancer screening of South African plants. J Ethnopharmacol 2008;119:455-61.
26. Saiah H, Allem R, Kebir FZ. Antioxidant and antibacterial activities of six Algerian medicinal plants. Int J Pharm Pharm Sci 2016;8:367-74.
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How to Cite
SINGH JASSAL, P., and M. SHARMA. “EVALUATION OF ANTIOXIDANT, ANTIBACTERIAL, ANTIHEMOLYTIC, AND PHYOCHEMICAL PROPERTIES OF FICUS BENJAMINA, FICUS INFECTORIA, AND FICUS KRISHNAE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Mar. 2019, pp. 68-73, doi:10.22159/ajpcr.2019.v12i3.25351.
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