@article{Rameshwar Naidu_Gunjan_CHEN_Sasidharan_2016, title={EVALUATION OF IN VITRO CYTOTOXIC ACTIVITY OF OCIMUM BASILICUM AND MENTHA SPICATA EXTRACTS}, volume={9}, url={https://journals.innovareacademics.in/index.php/ajpcr/article/view/10683}, abstractNote={<p>Objective: The objective of the current research was to evaluate the cytotoxicity of Ocimum basilicum (OB) and Mentha spicata (MS).<br />Methods: In vitro cytotoxic potential of OB and MS extracts was evaluated against human cancer HL60 cell lines using 3-[4,5-dimethylthiazol-2-yl]-2,5<br />diphenyl tetrazolium bromide assay. The treated cell morphology was observed by light microscope.<br />Results: The OB and MS extracts inhibited proliferation of HL60 cells in concentration dependent manner with ICâ‚…â‚€ of 45.67 µg/ml and 98.1 µg/ml<br />for 42 hrs, respectively. The morphology of the HL60 cells treated with OB and MS extracts at high concentrations (200 and 100 µg/ml) indicated cell<br />shrinkage, clumping, and signs of apoptosis.<br />Conclusion: OB and MS extracts induced cell death in HL60 cell lines. Further, in vivo studies and identification of active components from OB extract,<br />and their exact mechanism of action could be useful in designing new anticancer therapeutic agents.<br />Keywords: Cytotoxicity; HL60 cell; apoptosis; medicinal plant.</p>}, number={3}, journal={Asian Journal of Pharmaceutical and Clinical Research}, author={Rameshwar Naidu, Jegathambigai and Gunjan, Manish and CHEN, YENG and Sasidharan, Sreenivasan}, year={2016}, month={May}, pages={131–134} }