@article{Subramanian_Shanmugam_2016, title={EXTRACELLULAR AND INTRACELLULAR SYNTHESIS OF SILVER NANOPARTICLES}, volume={9}, url={https://journals.innovareacademics.in/index.php/ajpcr/article/view/13302}, DOI={10.22159/ajpcr.2016.v9s2.13302}, abstractNote={<p>ABSTRACT<br />Objective: The cellular synthesis of nanoparticle is a green process and alternative for a conventional process for the preparation of silver nanoparticles.<br />In our research, focus has been given to the development of an efficient and eco-friendly viable process for the synthesis of silver nanoparticles using<br />cancer and non-cancerous cells, a cell culture that was isolated. The results of this investigation are observed that silver nanoparticles could be<br />induced to synthesis intra- and extra-cellularly using mammalian cells such as cancerous and non-cancerous cells.<br />Methods: The silver nanoparticles are synthesized by the cancer and non-cancerous cells such as HeLa (Homo sapiens, human), SiHa, and human<br />embryonic kidney-293 cell lines. The silver nanoparticles were characterized by ultraviolet (UV)-visible spectroscopy, transmission electron<br />microscopy (TEM), and X-ray powder diffraction (XRD).<br />Results: The silver nanoparticles exhibited maximum absorbance at 415 nm in UV-visible spectroscopy. The XRD confirms the characteristic of the<br />crystal lattice of silver nanoparticles by observing three peaks: Peak at 38 is due to reflection from (111), peak at 44 is due to reflection from (200),<br />and peak at 65 is due to reflection from (220). TEM images showed the formation of stable silver nanoparticles in the cell lines.<br />Conclusion: The method of extraction of intracellular/extracellular synthesis of silver nanoparticles was inexpensive, simple, and effective in large<br />scale with no need to use of complex process equipment. The cancer cell considered as a biological factory at nanoscale dimension which continued to<br />grow after synthesis of silver nanoparticles. The silver reduction by these cancer cells has occurred through energy-dependent processes that lead to<br />the high output of this reaction. Hence, this new approach of using a mammalian cell for the successful synthesis of nanosized silvers could be easily<br />scaled up, which establishes its commercial viability and also useful in the drug delivery and drug targeting.<br />Keywords: Silver nanoparticles, Cancer cells, Biosynthesis and characteristics of silver nanoparticles.</p><p> </p>}, number={8}, journal={Asian Journal of Pharmaceutical and Clinical Research}, author={Subramanian, Prasanna and Shanmugam, Kirubanandan}, year={2016}, month={Oct.}, pages={133–139} }