@article{LESTARI_SOEMARDJI_Fidrianny_Yusuf_2017, title={THE CAPABILITY OF BRINE SHRIMP TEST AS A TERATOGENICITY SCREENING SYSTEM}, volume={10}, url={https://journals.innovareacademics.in/index.php/ajpcr/article/view/16511}, DOI={10.22159/ajpcr.2017.v10i3.16511}, abstractNote={<p>ABSTRACT<br />Objectives: The goals of this study were to analyze the capability of brine shrimp test (BST) as a potent teratogenicity screening system on teratogenic<br />agents (methotrexate, captopril, diclofenac, phenytoin, warfarin, and valproic acid).<br />Methods: Artemia cysts were hatched into 1st stage nauplii, then taken and put into seawater medium which contain test substance and kept alive until<br />2nd stage, 3rd stage, and 4th stage, and number of deaths, morphological abnormalities, body length, and retarded of development were observed for each stage.<br />Results: Hatch ability of cysts in methotrexate 0.015 mg/ml, captopril 0.25 mg/ml, diclofenac 0.075 mg/ml, phenytoin 1.56 mg/ml, and valproic acid<br />2.5 mg/ml were significantly different compared to control (p<0.05). Nauplii survival in methotrexate 0.015 mg/ml, captopril 0.25 mg/ml, diclofenac<br />0.075 mg/ml, phenytoin 1.56 mg/ml, and valproic acid 2.5 mg/ml were significantly different to control (p<0.05). The morphological abnormalities<br />was found in methotrexate 0.015 mg/ml, captopril 0.25 mg/ml. Nauplii with retarded development were expressed in methotrexate 0.015 mg/ml,<br />captopril 0.25 mg/ml, diclofenac 0.075 mg/ml, phenytoin 1.56 mg/ml, and valproic acid 2.5 mg/ml. Significant difference in body length was presented<br />in captopril 0.25 mg/ml, and phenytoin 1.56 mg/ml compared to control (p<0.05).<br />Conclusion: BST can be used as an alternative method of the teratogenic screening test, although not as sensitive teratogenic tests on mammals. This<br />screening method was not suitable for a compound which its chemical characteristic can change the tonicity of the medium.<br />Keywords: Brine shrimp test, Teratogenicity, Methotrexate, Captopril, Diclofenac, Phenytoin, Warfarin, Valproic acid.</p>}, number={3}, journal={Asian Journal of Pharmaceutical and Clinical Research}, author={LESTARI, METI WIDYA and SOEMARDJI, ANDREANUS A and Fidrianny, Irda and Yusuf, Ayda T}, year={2017}, month={Mar.}, pages={454–457} }