@article{AISHWARYA_VENKATESWARULUNAGAM_NETALA_TARTTE_2017, title={SCREENING AND IDENTIFICATION OF HEAVY METAL-TOLERANT ENDOPHYTIC FUNGI LASIODIPLODIA THEOBROMAE FROM BOSWELLIA OVALIFOLIOLATA AN ENDEMIC PLANT OF TIRUMALA HILLS}, volume={10}, url={https://journals.innovareacademics.in/index.php/ajpcr/article/view/16697}, DOI={10.22159/ajpcr.2017.v10i3.16697}, abstractNote={<p>ABSTRACT<br />Aim: The aim of this study was to evaluate the heavy metal resistance potentiality of endophytic fungi isolated from the leaves of Boswellia ovalifoliolata,<br />an endemic medicinal plant of Tirumala Hills.<br />Methods: Initially, isolation of fungal endophytes was carried out. Isolated fungi were screened for the heavy metal resistance against Co, Cd, Cu<br />and Zn using growth and evaluated their maximum tolerant capacity. Molecular identification of endophytic fungi was carried out by 18S rRNA gene<br />amplification and Sanger’s nucleotide sequencing. Phylogenetic tree was constructed using NCBI Clustal W.<br />Results: Ten different endophytic fungi were isolated from the leaves of B. ovalifoliolata. Among the isolated endophytic fungi, five showed resistance<br />to Co, Cd, Cu, and Zn. The most resistant fungus was identified as Lasiodiplodia theobromae based on 18S rRNA gene sequencing.<br />Conclusions: L. theobromae was isolated from B. ovalifoliolata and identified as one of the useful fungi involved in mycoremediation against heavy<br />metal toxicity.<br />Keywords: Heavy metals, Endophytic fungi, Endemic plant, Bioremediation.</p>}, number={3}, journal={Asian Journal of Pharmaceutical and Clinical Research}, author={AISHWARYA, SANI and VENKATESWARULUNAGAM and NETALA, VASUDEVA REDDY and TARTTE, VIJAYA}, year={2017}, month={Mar.}, pages={488–491} }