@article{Safriana_Rosidah_Hasibuan_Satria_2018, title={CYTOPROTECTIVE EFFECT OF ETHANOL FRACTION OF VERNONIA AMYGDALINA DEL. LEAVES AGAINST THE VERO CELLS}, volume={11}, url={https://journals.innovareacademics.in/index.php/ajpcr/article/view/26610}, DOI={10.22159/ajpcr.2018.v11s1.26610}, abstractNote={<p class="Default"> </p><p class="Pa9"> <strong>Objectives: </strong>The objective of this study is to assess the cytoprotective effect of ethanol fraction (EtF) of <em>Vernonia amygdalina </em>Del. leaves EtF against Vero cells which induced by hydrogen peroxide (H2O2).</p><p class="Pa9"><strong>Methods: </strong>Cytoprotective effects of EtF were analyzed by 3-(4,5-dimethylthiazolyl-2)2,5-dipheniltetrazolium bromide to Vero cells which induced by 0.8 mM H2O2, apoptosis was determined by flow cytometry, and reactive oxygen species (ROS) expression was analyzed by immunocytochemistry.</p><p class="Pa9"><strong>Results: </strong>EtF was showed the largest percentage viable cell (78.75±2.51%) at 50 μg/mL. In the analysis of apoptosis by flow cytometry was showed the percentage of viable cell count of 59.56% and EtF was decreased the expression of ROS.</p><p><strong>Conclusion: </strong>EtF has cytoprotective activity toward Vero cells induced by 0.8 mM H2O2.</p>}, number={13}, journal={Asian Journal of Pharmaceutical and Clinical Research}, author={Safriana and Rosidah and Hasibuan, Poppy Anjelisa Zaitun and Satria, Denny}, year={2018}, month={Apr.}, pages={214–216} }