@article{TO_N_CO_2020, title={IDENTIFICATION OF POTENTIAL ANTIOXIDANT AND HEPATOPROTECTIVE CONSTITUENTS OF VITEX DONIANA BY UHPLC/+ESI-QQTOF-MS/MS ANALYSIS}, volume={13}, url={https://journals.innovareacademics.in/index.php/ajpcr/article/view/37956}, DOI={10.22159/ajpcr.2020.v13i8.37956}, abstractNote={<p><strong>Objective: </strong>This study evaluated the antioxidant and hepatoprotective activities of the dichloromethane (DCM-F) and <em>n</em>-butanol (BUT) fractions of <em>Vitex doniana </em>and identified the bioactive constituents using liquid chromatography-mass spectrometry (LC-MS) analysis.</p> <p><strong>Methods: </strong>The methanol extract of the leaves was subjected to liquid-liquid fractionation and the BUT soluble separated by vacuum LC (VLC) using binary mixtures of DCM in methanol (1 L) sequentially in the ratios of 9:1 BF1–1:9 BF5. The antioxidant activity was investigated <em>in vitro </em>using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, phosphomolybdenum total antioxidant capacity, and ferric ion reducing antioxidant power assay methods. The <em>in vivo </em>antioxidant potential was performed by measuring the concentrations of both enzymatic and non-enzymatic parameters in carbon tetrachloride (CCl4)-induced hepatic injury.</p> <p><strong>Results: </strong>The extract, DCM-F, and the VLC sub-fractions showed good antioxidant activity which is comparable with ascorbic acid (53.6%). DCM-F showed the highest antioxidant activity (71.6%), while the least activity was shown by BF5 (15.0%). Peak dereplication of LC-MS chromatograms identified several putative compounds in the BUT soluble. DCM-F caused a significant increase (p<0.05) in superoxide dismutase, catalase, glutathione peroxidase, and Vitamins C and E in CCl4-induced rats compared to standard. The levels of serum alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase, and malondialdehyde that were elevated in CCl4-induced rats were also significantly reduced (p<0.05) by the DCM-F compared to standard.</p> <p><strong>Conclusion: </strong>The ability of <em>V. doniana </em>leaves to mop up free radicals and reverse the CCl4-induced hepatic injury in rats suggests antioxidant potentials. The bioactive compounds identified in BUT fraction could support its ethnomedicinal uses in the treatment of illnesses associated with radical scavenging activity.</p>}, number={8}, journal={Asian Journal of Pharmaceutical and Clinical Research}, author={TO, AYOKA and N, NWACHUKWU and CO, NNADI}, year={2020}, month={Aug.}, pages={142–148} }