@article{C_A_2015, title={DISCOVERY OF POLYGALACTURONASE PRODUCING BACILLUS TEQUILENSIS STRAIN ARMATI USING 16S rRNA GENE SEQUENCING}, volume={8}, url={https://journals.innovareacademics.in/index.php/ajpcr/article/view/4906}, abstractNote={<p>Objective: To identify a novel strain of polygalacturonase producing Bacillus tequilensis from poultry farm.<br />Methods: Poultry feces sample was serially diluted, and the pure isolate was subjected to morphological tests, biochemical tests, genomic DNA<br />isolation, polymerase chain reaction amplification, amplicon purification and 16S rRNA gene sequencing. The strain was screened for polygalacturonase<br />production using plate assay method. The nucleotide sequences obtained from the isolate were subjected to BLAST tool for the pairwise alignment.<br />RNA secondary structure was predicted through RNAStructure Web Server. Multiple antibiotic resistance (MAR) index was determined against six<br />antibiotics through disc diffusion method. Phylogenetic trees were inferred using the neighbor-joining algorithm in Molecular Evolution Genetic<br />Analysis software version 4.0.<br />Results: B. tequilensis strain ARMATI showed the production of polygalacturonase. MAR index of this novel strain was found to be zero. RNA<br />secondary structure with a minimum free energy of −281.40 kcal/mol was obtained. Phylogenetic tree of Bacillus 16S rRNA genes separate each<br />Bacillus according to their taxonomic positions and were supported statistically.<br />Conclusion: The present investigation clearly indicates the isolation, molecular characterization and sequence analysis of B. tequilensis strain ARMATI<br />from poultry farm, and industrial application of this novel strain for the production of the enzyme.<br />Keywords: Bacillus tequilensis, Multiple antibiotic resistance index, Polygalacturonase, Phylogenetic tree, RNA secondary structure, RNAStructure<br />Web Server.</p>}, number={5}, journal={Asian Journal of Pharmaceutical and Clinical Research}, author={C, AARTI and A, KHUSRO}, year={2015}, month={Sep.}, pages={58–62} }