@article{Pamudji_2015, title={MICROEMULSION FORMULATION OF ALOE VERA GEL AND APIUM GRAVEOLENS ETHANOL EXTRACT FOR OPTIMIZING HAIR GROWTH PROMOTION}, volume={8}, url={https://journals.innovareacademics.in/index.php/ajpcr/article/view/6926}, abstractNote={<p>ABSTRACT<br />Objective: Follicular penetration of hair promotion substances to the bulge region is needed to potentiate cell proliferation and hair shaft lengthening.<br />Nano-size globule is one of important delivery systems applied for hair follicle targeting. This research aims to explore the effect of microemulsion<br />on hair promoting activities of a mixture of hydrophilic substances of Aloe vera gel and semipolar substances of Apium graveolens ethanolic extract.<br />Methods: The clear water in oil (W/O) and oil in water (O/W) microemulsions were developed by constructing phase diagram of various ratios<br />of Crodamol GTCC, Croduret 50 SS, glycerin/alcohol, and water. A. vera (1%) and celery extract (2%) were incorporated during microemulsion<br />preparation. The microemulsions were applied over shaved skin areas of the backs of Wistar rats, and the average length of 10 hair samples was<br />determined at day 7, 14, and 21 compared to the un-treated area. The weight of hair/cm2 area was also analyzed at day 21. Hair growth promotion of<br />the microemulsions was compared to the other groups of rats applied by A. vera in water (AVW), celery extract in water and oil (CEW and CEO), and<br />combination of A. vera and celery extract in water (AVCEW).<br />Result: The W/O and O/W microemulsion consisted of globules at the average size of 61.7 and 81.5 nm, respectively, which showed stable size<br />following six cycles of freeze-thaw. The W/O microemulsion increased hair growth dramatically started at day 7 (220%) and day 14 (275%) and<br />slowed-down at day 21 (163%) with similar profile shown by AVW (74.5, 65.8, 31.7%, respectively). On the contrary, the hair growth promotion of<br />O/W microemulsion delayed to day 14 but maintained till day 21 (143.7, 214.5, 171.9 at day 7, 14, and 21, respectively) with similar profile shown<br />by CEW and CEO. Hair densities were also doubled in the areas treated by both microemulsion types than in the areas treated by a combination of<br />AVCEW in water.<br />Conclusion: Incorporation of hydrophilic and semipolar substances of hair growth promoter in nano-size globules of W/O and O/W microemulsions<br />lead to pilosebaceous targeting and optimize the growth promotion activities.<br />Keywords: Aloe vera, Apium graveolens, Water in oil microemulsion, Oil in water microemulsion, Hair growth, Pilosebaceous.</p>}, number={4}, journal={Asian Journal of Pharmaceutical and Clinical Research}, author={Pamudji, Jessie Sofia}, year={2015}, month={Jul.}, pages={319–323} }