TY - JOUR AU - Rameshwar Naidu, Jegathambigai AU - Gunjan, Manish AU - CHEN, YENG AU - Sasidharan, Sreenivasan PY - 2016/05/01 Y2 - 2024/03/28 TI - EVALUATION OF IN VITRO CYTOTOXIC ACTIVITY OF OCIMUM BASILICUM AND MENTHA SPICATA EXTRACTS JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 9 IS - 3 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/10683 SP - 131-134 AB - <p>Objective: The objective of the current research was to evaluate the cytotoxicity of Ocimum basilicum (OB) and Mentha spicata (MS).<br />Methods: In vitro cytotoxic potential of OB and MS extracts was evaluated against human cancer HL60 cell lines using 3-[4,5-dimethylthiazol-2-yl]-2,5<br />diphenyl tetrazolium bromide assay. The treated cell morphology was observed by light microscope.<br />Results: The OB and MS extracts inhibited proliferation of HL60 cells in concentration dependent manner with IC₅₀ of 45.67 µg/ml and 98.1 µg/ml<br />for 42 hrs, respectively. The morphology of the HL60 cells treated with OB and MS extracts at high concentrations (200 and 100 µg/ml) indicated cell<br />shrinkage, clumping, and signs of apoptosis.<br />Conclusion: OB and MS extracts induced cell death in HL60 cell lines. Further, in vivo studies and identification of active components from OB extract,<br />and their exact mechanism of action could be useful in designing new anticancer therapeutic agents.<br />Keywords: Cytotoxicity; HL60 cell; apoptosis; medicinal plant.</p> ER -