TY - JOUR AU - Kusmardiyani, Siti AU - Alfianti, Fitria AU - Fidrianny, Irda PY - 2016/07/01 Y2 - 2024/03/29 TI - ANTIOXIDANT PROFILE AND PHYTOCHEMICAL CONTENT OF THREE KINDS OF LEMON GRASS GROWN IN WEST JAVA-INDONESIA JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 9 IS - 4 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/12578 SP - 381-385 AB - <p>ABSTRACT<br />Objective: The aims of this research were to determine antioxidant activity from various herbs extracts of three kinds of lemon grass using two<br />antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) and correlation of total<br />phenolic content (TPC), total flavonoid content (TFC), and total carotenoid contents (TCC) with their inhibitory concentration 50% (IC<br />) of DPPH and<br />exhibitory concentration 50% (EC<br />50<br />) of FRAP.<br />Methods: A sample was extracted by reflux method using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant<br />activities using DPPH and FRAP assays, determination of TPC, TFC, and TCC were carried out by ultraviolet -visible spectrophotometry and correlation<br />with their IC<br />50<br /> of DPPH and EC<br />50<br /> of FRAP capacities were analyzed by Pearson's method.<br />Results: The ethanolic herbs extract of Cymbopogon citratus (CC) had the lowest IC<br />50<br />50<br /> of DPPH scavenging activity 2.75 µg/ml and the lowest EC</p><p>of FRAP capacity 12.22 µg/ml. Ethanolic herbs extract of Cymbopogon winterianus exposed the highest phenolic content and its n-hexane extract<br />presented the highest carotenoid content. Ethyl acetate herbs extract of CC gave the highest flavonoid content. There was significantly negative<br />correlation between TPC in CC herbs extract with their IC<br />50<br /> of DPPH and EC<br />50<br /> of FRAP.<br />Conclusions: All herbs extracts from three kinds of lemon grass were categorized as a very strong antioxidant by DPPH method. Phenolic compounds<br />in CC were the major contributor in antioxidant activities by DPPH and FRAP methods. DPPH and FRAP gave linear result in antioxidant activities of<br />herbs extract of three kinds of lemon grass.<br />Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ferric reducing antioxidant power, Lemon grass, Three kinds, Herbs.<br />50</p> ER -