TY - JOUR AU - El Wakeel, Maged A. AU - Abou-el-asrar, Mohamed AU - EL-KASSAS, GHADA M. AU - Elabd, Mona A AU - Abu Zeid, Dina AU - Sabry, Rania N AU - Awadallah, Eman PY - 2017/10/01 Y2 - 2024/03/29 TI - URINARY MARKERS OF OXIDATIVE DNA DAMAGE IN TYPE 1 DIABETIC CHILDREN: RELATION TO MICROVASCULAR COMPLICATIONS JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 10 IS - 10 SE - Original Article(s) DO - 10.22159/ajpcr.2017.v10i10.18930 UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/18930 SP - 318-322 AB - <p class="Default"> </p><p class="Pa6"> <strong>Objective: </strong>Type 1 diabetes mellitus (T1DM) is a widespread metabolic disease, which frequently carries with it a significant impact on human health. Oxidative damage and tissue inflammation have been claimed to be a typical pathogenic component for the progression of diabetic complications. We aim in this study to explore the relation of urinary 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) (as a marker of nucleic acid oxidation) to microvascular complications in T1DM.</p><p class="Pa6"><strong>Methods: </strong>A case–control study, enrolling 45 T1DM children and an equivalent number of healthy subjects, was performed. Full clinical examination and anthropometric measurement were performed to all subjects. Urinary assessment for 8-oxodG and albumin was done in addition to blood sampling for lipid profile and glycated Hb (HbA1c) assay. Complete ocular examination for assessment of diabetic retinopathy (DR) was also done.</p><p class="Pa6"><strong>Results: </strong>Levels of urinary 8-oxodG, serum cholesterol, triglycerides, and low-density lipoprotein in cases were significantly higher than non-diabetics; these levels were likewise higher in uncontrolled T1DM patients in comparison with well-controlled T1DM subjects. Urinary 8-oxodG and HbA1c were significantly higher in diabetic patients with albuminuria and DR compared to patients without complications. Significant positive correlation was found between 8-oxodG with HbA1c (r=0.8, p&lt;0.01), diastolic blood pressure (DBP) (r=0.4, p=0.02), and cholesterol (r=0.4, p=0.05).</p><p><strong>Conclusion: </strong>Urinary 8-oxodG was found to be a reliable marker for assessing oxidative DNA damage in T1DM and can be used in the determination of microvascular complications related to diabetes.</p> ER -