TY - JOUR AU - Gm, Subaiea AU - M, Aljofan AU - Vr, Devadasu AU - Tm, Alshammari PY - 2017/11/01 Y2 - 2024/03/28 TI - ACUTE TOXICITY TESTING OF NEWLY DISCOVERED POTENTIAL ANTIHEPATITIS B VIRUS AGENTS OF PLANT ORIGIN JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 10 IS - 11 SE - Original Article(s) DO - 10.22159/ajpcr.2017.v10i11.20717 UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/20717 SP - 210-213 AB - <p class="Pa7"><strong>Objective: </strong>Our previous studies indicate that alkaloids could be developed as potential antihepatitis B agents. In the present study, we investigated the <em>in vitro </em>antihepatitis B virus (HBV) activity and <em>in vivo </em>acute oral toxicity of three isoquinoline alkaloids [-(-) Canadine, Corydadine, and Berberine] obtained from <em>Fumaria </em>and <em>Corydalis </em>species. The compounds were selected based on their therapeutic indexes calculated previously <em>in vitro</em>.</p><p class="Pa7"><strong>Methods: </strong>The antiviral activity and cytotoxicity of selected isoquinoline alkaloids were evaluated <em>in vitro </em>in HepG2 cells. <em>In vivo, </em>acute oral toxicity was performed in female mice following the Organization for Economic Cooperation and Development test guideline-423 (acute toxicity class method).</p><p class="Pa7"><strong>Results: </strong>The selected agents have shown high antiviral activity against HBV and low cytotoxicity <em>in vitro</em>. The results obtained from an acute oral toxicity study revealed that the LD50 of all the test compounds was &gt;2000 mg/kg when administered orally to mice. All the tested compounds fall under the category 5 (unclassified) according to the Globally Harmonized System, with a LD50 value &gt;2000 mg/kg when orally administered to mice.</p><p><strong>Conclusion: </strong>The results of the study revealed that OR-13 and MNAD can be studied further and can be developed as antihepatitis B drugs.</p> ER -