TY - JOUR AU - Melani Maskoen, Ani AU - L Nasroen, Saskia AU - Nanda Fauziah, Prima AU - Setiawan Soeria Soemantri, Eky AU - A Gani, Basri PY - 2018/04/01 Y2 - 2024/03/29 TI - EXPRESSION OF TRANSFORMING GROWTH FACTOR ALPHA BAMHI AND RSAI GENE VARIANTS ASSOCIATED WITH NON-SYNDROMIC CLEFT PALATE OF INDONESIAN SUBJECTS USING POLYMERASE CHAIN REACTION METHOD JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 11 IS - 4 SE - Original Article(s) DO - 10.22159/ajpcr.2018.v11i4.24242 UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/24242 SP - 351-354 AB - <p> <strong>Objective: </strong>This study aimed to detect and analyze transforming growth factor alpha (<em>TGFA</em>) <em>BamH</em>I and <em>Rsa</em>I gene variants which associated with the risk factor of non-syndromic cleft palate only (NS CPO) of Indonesian subject.</p><p><strong>Methods: </strong>This was case-control study using samples from 32 NS CPO subjects and 28 control subjects. DNA was extracted from venous blood, and the <em>TGFA </em>gene was amplified using polymerase chain reaction technique, then digestion product from <em>Taq</em>I and <em>Rsa</em>I restriction enzyme was evaluated. Statistical analysis to determine significant differences of gene variant frequency among NS CPO subject and control was <em>χ</em>2. The odds ratio (OR) was used to determine a risk factor of NS CPO.</p><p><strong>Results: </strong>The study results showed that the <em>TGFA BamH</em>I gene variant was not identified in NS CPO among Indonesian but <em>TGFA Rsa</em>I gene variant was identified. The frequency of TT/B1B1 homozygous mutant genotype was 80.0% in NS CPO subjects and 20.0% in control subjects (OR=3.857; 95% confidence interval=0.405–36.749).</p><p><strong>Conclusion: </strong><em>TGFA Rsa</em>I gene can be considered a risk factor of NS CPO compared <em>TGFA BamH</em>1 gene of Indonesian subjects.</p> ER -