TY - JOUR AU - KANNAYIRAM, GOMATHI AU - A, SANDHYA AU - S, SOWMIYA AU - S, VALARMATHI AU - JOSEPH, DANIEL PY - 2019/02/07 Y2 - 2024/03/29 TI - ANTI-INFLAMMATORY ACTIVITY OF NIGELLA SATIVA SILVER NANOPARTICLES: BIOCHEMICAL STUDY JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 12 IS - 2 SE - Original Article(s) DO - 10.22159/ajpcr.2019.v12i2.29775 UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/29775 SP - 346-349 AB - <p><strong>Objective: </strong>The aim of this study is to evaluate the anti-inflammatory activity of <em>Nigella sativa </em>silver nanoparticles (NS AgNPs).</p><p><strong>Methods: </strong>Fourier transform infrared analysis was used to characterize the NS AgNPs and the extract. 2,2-diphenylpicrylhydrazyl assay was done to test the antioxidant potency of NS AgNP. Furthermore, <em>in vitro </em>anti-inflammatory activity of the extract and the NS AgNP was determined by red blood cell (RBC) membrane stabilization assay, protein inhibition assay, and interleukin-1 (IL-1) beta assay.</p><p><strong>Results: </strong>The NS AgNP exhibited dose-dependent antioxidant property. At the concentration 0.01 mg/ml 80% of radical was scavenged by NS AgNP. Inhibition of protein denaturation assay also suggests that NS AgNP shows the highest activity (70%) when compared with the standard drug aspirin (65%). RBC assay suggests that NS AgNP stabilizes the RBC membrane and prevents leaking. In the enzyme-linked immunosorbent assay method the NS AgNP showed better IL-1 beta inhibition activity when compared to aqueous extract.</p><p><strong>Conclusion: </strong>From the study, it was inferred that NS AgNPs are more effective when compared to the extract. These results suggest that NS AgNP can be used to treat inflammatory disorders.</p> ER -