TY - JOUR AU - P, ANITHA AU - TH, NAZEEMA PY - 2020/04/07 Y2 - 2024/03/29 TI - IN VITRO EVALUATION OF ENZYMIC ANTIOXIDANTS AND FREE RADICAL SCAVENGING ACTIVITY IN THE ETHANOLIC EXTRACT OF CRESCENTIA CUJETE LEAVES JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 13 IS - 4 SE - Original Article(s) DO - 10.22159/ajpcr.2020.v13i4.36794 UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/36794 SP - 92-95 AB - <p><strong>Objectives: </strong>This study aims to evaluate the enzymic antioxidants and free radical scavenging present in the ethanolic leaf extracts of <em>Crescentia cujete</em>.</p><p><strong>Methods: </strong>Enzymic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase, glutathione peroxidase, and glutathione S-transferase (GST) were estimated by standard methods. Free radical scavenging potential was evaluated by diphenylpicrylhydrazyl (DPPH), nitric oxide, and hydroxyl radical methods using an ethanolic extract of <em>C. cujete </em>leaf.</p><p><strong>Results: </strong>The leaf extract of <em>C. cujete </em>showed the maximum activity of CAT, SOD, GST, glutathione reductase, and peroxidase activity. CAT activity was formed to be highest in the ethanolic extract of <em>C. cujete </em>leaf. DPPH radical scavenging activity was reported as 38.5 μg/ml, nitric oxide was found to be 200.77 μg/ml, and hydroxyl radical scavenging exhibited 108.42 μg/ml normalized with ascorbic acid.</p><p><strong>Conclusion: </strong>From the results, it has concluded that the ethanol extract of the <em>C. cujete </em>leaf has a prospective source of natural antioxidant that would be a great significance as therapeutic agents in preventing or slowing the progress of reactive oxygen species and related oxidative stress-related degenerative diseases.</p> ER -