TY - JOUR AU - NURYANA, CH. TRI AU - MUBARIKA, SOFIA AU - WIROHADIDJOJO, YOHANES WIDODO AU - ARFIAN, NUR AU - PUTUMEGAADITYA DEVI AYU MARA, AU - FAIZ HUWAIDI, AHMAD PY - 2020/05/07 Y2 - 2024/03/29 TI - ACHATINA FULICA MUCUS ATTENUATES ULTRAVIOLET B-INDUCED FIBROBLAST PHOTOAGING THROUGH REDUCING INFLAMMATION, ANGIOGENESIS, AND MATRIX METALLOPROTEINASE JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 13 IS - 5 SE - Original Article(s) DO - 10.22159/ajpcr.2020.v13i5.37284 UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/37284 SP - 199-203 AB - <p><strong>Objective: </strong>This study aimed to observe the effects of <em>Achatina fulica </em>mucus (AFM) on ultraviolet B (UVB)-induced fibroblast photoaging by assessing monocyte chemotactic protein (MCP)-1, vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMP)-3, and MMP-12 mRNA expressions.</p><p><strong>Methods: </strong>Cell cultures of normal human dermal fibroblasts (NHDFs) were divided into six groups: Group 1 was normal fibroblasts without UVB irradiation as normal control and Groups 2–5 consisted of 100 mJ/cm2 UVB-induced aged fibroblasts. Group 2 had no treatment as negative control, Group 3 was treated by platelet-rich plasma 10% as positive control group, and Groups 4–6 were treated by various concentrations of AFM (3.9 μl, 15.625 μl, and 62.5 μl). The MCP-1, VEGF, MMP-3, and MMP-12 mRNA expressions in the different NHDF groups were assessed by quantitative polymerase chain reaction.</p><p><strong>Results: </strong>The mRNA expressions of MCP-1, VEGF, MMP-3, and MMP-12 in the AFM group compared to the UVB group decreased 8, 5, 5, and 4 folds, respectively. AF62 exhibited the highest improvement among the other AFM-treated groups.</p><p><strong>Conclusion: </strong>AFM treatment attenuates UVB-induced fibroblasts photoaging by reducing inflammation, angiogenesis, and matrix metalloproteinases.</p> ER -