TY - JOUR AU - BINO, JAMES D TERISH AU - Kannappan, AU - Jith, Sasi AU - Kumar, Suresh PY - 2015/01/01 Y2 - 2024/03/29 TI - SIMULTANEOUS ESTIMATION AND METHOD DEVELOPMENT FOR L-CARNITINE AND METFORMIN IN HUMAN PLASMA USING LC-MS/MS JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 8 IS - 1 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/3897 SP - 185-191 AB - <p><strong> </strong></p><p>Objectives: The objective was to develop a robust simultaneous method for quantifying L-Carnitine and metformin using liquid chromatography<br />mass spectrometer (LCMSMS).<br />Methods: LCMSMS method was developed considering its selectivity, sensitivity, and accuracy. Blank correction method was followed as L-Carnitine<br />is found endogenously. Range was selected based on the Normal value for L-Carnitine and maximum concentration for metformin.<br />Results: Rugged simultaneous method using ultra flow LC mass spectrometer for the estimation of L-Carnitine and metformin, in human plasma<br />with low limit of quantification and upper limit of quantification of 2.289 μg/mL and 33.675 μg/mL for L-Carnitine, 43.483 ng/ml and 639.450 ng/ml<br />for metformin, respectively, was developed which can be used for therapeutic drug monitoring and as a prognostic tool for Type 2 diabetes mellitus<br />(T2DM). Extraction was optimized using Protein precipitation (PP) in which sample clean-up was simple, and recovery was consistent. Linearity was<br />checked and found to be within acceptance criteria with 1/×2 as the weighing factor. As L-Carnitine is present endogenously blank correction was<br />carried by injecting blank six times and calculated for normalization. As the extraction is by simple PP internal standard was not used, and method is<br />meeting the criteria for validation using LCMSMS.<br />Conclusion: Hence, considering L-Carnitine as the biomarker for T2DM and metformin as the drug for treatment, a simultaneous method was<br />developed which can be validated as per regulatory requirements and can be verified for the applicability of the method as a therapeutic drug<br />monitoring and prognostic tool.<br />Keywords: L-Carnitine, Metformin, Type 2 diabetes, Liquid chromatography mass spectrometer.</p> ER -