TY - JOUR AU - RAJPUT, KAMNI AU - CHANDRA DUBEY, RAMESH PY - 2021/03/07 Y2 - 2024/03/29 TI - IN VITRO ANTIOXIDANT AND IN VIVO ANTIDIABETIC ACTIVITY OF TWO POTENTIAL PROBIOTIC ENTEROCOCCUS SPP. ON ALLOXAN-INDUCED DIABETIC RATS JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 14 IS - 3 SE - Original Article(s) DO - 10.22159/ajpcr.2021.v14i3.40321 UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/40321 SP - 94-98 AB - <p><strong>Objective: </strong><em>In vitro </em>antioxidant activity, <em>in vivo </em>antidiabetic property and intestinal attachment by two potential probiotic bacterial strains, namely, <em>Enterococcus faecium </em>and <em>Enterococcus hirae </em>were studied using albino rats.</p><p><strong>Methods: </strong>Antioxidant the activity was assessed using 2,2-Diphenyl-1-picrylhydrazyl radicals scavenging assay. Alloxan was administered intraperitoneally to induce diabetic conditions in experimental rats. Animals were treated with oral administration of <em>Enterococcus </em>spp., such as <em>E. faecium, </em>and <em>E. hirae </em>isolated from goat and sheep milk. The control animal group received normal saline for the same days. Glibenclamide drug was used as a positive control against probiotic bacterial cells.</p><p><strong>Results: </strong>However, administration of probiotic bacterial strains <em>E. faecium </em>and <em>E. hirae</em>, in albino rats significantly (p&lt;0.05) at varying doses lowered blood glucose levels in diabetic rats as compared to the diabetic control group. Both the species of <em>Enterococcus </em>increased the bodyweight of experimental rats. However, <em>E. faecium </em>was the best antidiabetic strain having the antioxidant activities also in comparison to <em>E. hirae</em>. The attachment of probiotic bacterial cells <em>E. faecium </em>on the rat’s intestine wall against pathogens was examined. Furthermore, <em>E. faecium </em>showed its aggregation with pathogens by attachment of the intestines of albino rats. This showed that both the bacterial strains exhibited <em>in vivo </em>antidiabetic effect.</p><p><strong>Conclusion: </strong>The results of this study showed that probiotic bacteria possess antioxidant, antidiabetic activities, and attachment of intestine.</p> ER -