TY - JOUR AU - Barbade, Komal D AU - Datar, Ajit G PY - 2015/03/01 Y2 - 2024/03/29 TI - ANTIBACTERIAL ACTIVITY, FREE RADICAL SCAVENGING POTENTIAL, PHYTOCHEMICAL INVESTIGATION AND IN-VIVO TOXICITY STUDIES OF MEDICINAL PLANT Embelia basaal (R. & S.) A. DC. JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 8 IS - 2 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/4210 SP - 171-177 AB - <p><strong> </strong></p><p>Objective: Present extensive study on medicinal plant Embelia basaal (R. &amp; S.) A. DC. was undertaken for evaluation and quantification of broad<br />spectrum antibacterial activity, free radical scavenging potential, phytochemical investigation, development of high performance thin layer<br />chromatography (HPTLC) fingerprint profile and in-vivo safety of the plant.<br />Methods: Dried fruits of the plant were powdered and macerated in variety of solvents to obtain four extracts of different polarities. Antibacterial<br />activity was evaluated using agar well diffusion assay. Most potent antibacterial extract of E. basaal was selected for further extensive studies.<br />Minimum inhibitory concentration (MIC) values of the potent antibacterial extract were determined using broth macrodilution method as per Clinical<br />Laboratory Standards Institute guidelines. Free radical scavenging activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl assay. Qualitative<br />phytochemical tests, quantification of total phenolic contents and HPTLC fingerprint analysis were carried out for phytochemical investigation. The<br />in-vivo safety of the plant was determined by conducting acute oral toxicity studies in mice as per Organization for Economic Co-operation and<br />Development guidelines test no. 420.<br />Results: Ethanol extract of E. basaal was found potent bactericidal against selected pathogens and chosen for further extensive studies. The extract<br />exhibited significant free radical scavenging activity (inhibitory concentration 50 value=50 μg/mL). Phytochemical investigation revealed presence<br />of phenolic compounds, terpenoids, tannins and alkaloids in the test extract and it was found safe in-vivo at a dose level of 2000 mg/kg body weight<br />of an animal.<br />Conclusion: E. basaal was found medicinally active in the present study. Its broad spectrum antibacterial activity, significant free radical scavenging<br />potential and in-vivo safety will provide a platform for advance studies in the area.</p><p>Keywords: Embelia basaal (R. &amp; S.) A. DC., Bactericidal activity, Free radical scavenging activity, Phytochemical investigation, High performance thin<br />layer chromatography fingerprint, Acute oral toxicity.</p> ER -