TY - JOUR AU - A, SURESH AU - B, MEENA AU - ROSE, SUMIT AU - R, MANI PY - 2015/09/01 Y2 - 2024/03/29 TI - INDUCTION OF METALLOTHIONEIN WITH CADMIUM CHLORIDE IN A ECONOMICALLY IMPORTANT FRESHWATER FISH-GRASS CARP, CTENOPHARYNGODON IDELLA (VALENCIENNES, 1844) JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 8 IS - 5 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/6941 SP - 276-281 AB - <p>Objectives: Metallothioneins (MTs) have been widely considered for their potential use as a specific biomarkers to reflect the existence of heavy<br />metal pollution, because their induction has been observed to be obviously elevated after heavy metal exposure in a large number aquatic<br />organisms. However, relatively fewer efforts have been made to study the related effects of MT in fish species, such as Ctenopharyngodon idella<br />(C. idella), a globally important aquaculture species. The objective of this study was to determine the cadmium (Cd) levels and MT induction in<br />muscle, gill, kidney, and liver in C. idella during acute Cd exposure, to study the relationship between tissue-specific Cd accumulation and MT<br />induction.<br />Methods: Cd accumulation and MT induction levels was determined according to the methods of Ma et al., 2007. Cd concentration was determined<br />using an atomic absorption spectrophotometer (Perkin Elmer Optima-5300 DV).<br />Results: The MT induction levels were found in an increasing order of liver&gt;kidney&gt;gill &gt;muscle. A positive correlation was shown between MT<br />induction and Cd accumulation. The accumulation of Cd levels in all tissues is distinct by time-dependent and dose-dependent.<br />Conclusion: These results suggest that MT in the liver played an important role to detoxify high quantities of Cd. Cd accumulation showed a positive<br />correlation with MT induction in all the tissues studied. Hence, the present investigation marks that MT levels can be considered as a biomarker for<br />acute waterborne Cd.<br />Keywords: Heavy metals, Atomic absorption spectrophotometry, Biomarker protein, Metallothionein, Ctenopharyngodon idella.</p> ER -