TY - JOUR AU - Fidrianny, Irda AU - Fikayuniar, Lia AU - Insanu, Muhamad PY - 2015/09/01 Y2 - 2024/03/29 TI - ANTIOXIDANT ACTIVITIES OF VARIOUS SEED EXTRACTS FROM FOUR VARIETIES OF RAMBUTAN (Nephelium lappaceum) USING DPPH AND ABTS ASSAYS JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 8 IS - 5 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/7350 SP - 215-219 AB - <p>Objectives: The objectives of this research were to study antioxidant activities from various seed extracts of four varieties of rambutan using<br />2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) methods and correlation of total flavonoid<br />content (TFC), total phenolic content (TPC), and total carotenoid content (TCC) with inhibitory concentration 50 (IC<br />50<br />) of DPPH and IC<br />Methods: Antioxidant activities, TFC, TPC, and TCC of various seed extracts were performed by ultraviolet -visible spectrophotometry and their<br />correlation with IC<br />50<br /> of DPPH and IC<br />50<br /> of ABTS antioxidant activities were analyzed by Pearson's method.<br />Results: Ethanolic seed extract of lebak bulus variety had the lowest IC<br /> of DPPH scavenging activity 7 µg/ml, while ethyl acetate seed extract of rajah<br />variety had the lowest IC<br />50<br />50<br /> of ABTS scavenging activity 7.34 µg/ml. The highest TFC, TPC, and TCC were given by ethyl acetate extract of binjai variety,<br />ethanolic extract of rapiah variety, and ethanolic extract of rajah variety, respectively. There were negatively high correlation between TFC, TPC,, TCC<br />in seed extracts of rapiah variety with their IC<br />50<br /> of DPPH and IC<br />50<br /> of ABTS scavenging activities.<br />Conclusions: All of ethyl acetate and ethanolic seed extracts of four varieties of rambutan were very strong antioxidant by DPPH and ABTS methods.<br />Flavonoid, phenolic, and carotenoid compound in seed extracts of rapiah variety were contributor in their antioxidant activities by DPPH and ABTS<br />methods. The IC<br />50<br /> of DPPH and IC<br />50<br /> of ABTS scavenging activities in seed extracts of lebak bulus, rajah, and rapiah varieties gave linear result.<br />Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid, Seed, Four varieties, Rambutan,<br />Flavonoid, Phenolic, Carotenoid.</p> ER -