TY - JOUR AU - Suniarti, Dewi Fatma AU - Soekanto, Sri Angky AU - Soedarsono, Nurtami AU - Abbas, Basril PY - 2015/09/01 Y2 - 2024/03/29 TI - THE EFFECT OF CHITOSAN ON OSTEOCLAST VIABILITY, BONE RESORPTION AND RADICAL OXYGEN PRODUCTION OF PRIMARY OSTEOCLAST CULTURE OF MOUSE BONE JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 8 IS - 5 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/7400 SP - 282-286 AB - <p>Objective: The purpose of this investigation was to determine the effect of chitosan on osteoclast cells by observing cell viability, bone resorption,<br />and radical oxygen species (ROS) production.<br />Methods: Osteoclast cells were obtained from the primary culture of bone marrow mouse. The osteoclast cells were identified by tartrate-resistant<br />acid phosphatase (TRAP) marker both on the cells and the culture medium. The osteoclast cell viability was observed with (3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium<br />bromide)<br />tetrazolium<br />assay<br />and Bradford<br />assay<br />for<br />total<br />protein<br />medium<br />culture,<br />while<br />ROS<br />production<br />was<br />measured</p><p>with<br />malondialdehyde<br />(MDA)<br />assay.<br />Slices<br />of cow<br />cortical<br />bone<br />were<br />used as a substrate<br />for<br />osteoclastic<br />resorption<br />and concentrated<br />hydrochloric</p><p>acid<br />were<br />used to<br />activate<br />resorption<br />and pit formation<br />by<br />any<br />osteoclasts.<br />Results: Osteoclast cells were identified by TRAP marker and chitosan treated group cells showed lower optical density value compared to control<br />(p&lt;0.05) on TRAP assay medium culture. Cell viability indicated lower on chitosan group than control (p&lt;0.05). There was a qualitative difference of<br />the pits formed on the bone surface between the control and the chitosan group. There was a significant difference in MDA (mmol/ml) between the<br />control and the chitosan group with (p&lt;0.05).<br />Conclusion: Based on this research, we conclude that chitosan inhibits the viability of osteoclast cells, decreases ROS production and bone resorption.<br />Keywords: Chitosan, Osteoclast proliferation, Radical oxygen species, Bone resorption.</p> ER -