TY - JOUR AU - R, Sampath AU - Saravanan, Renuka AU - Pemiah, Brindha AU - Ramalingam, Sivakumar PY - 2016/01/01 Y2 - 2024/03/28 TI - THROMBOLYTIC ACTIVITY OF Punica granatum FRUIT AND PEEL EXTRACT JF - Asian Journal of Pharmaceutical and Clinical Research JA - Asian J Pharm Clin Res VL - 9 IS - 1 SE - Original Article(s) DO - UR - https://journals.innovareacademics.in/index.php/ajpcr/article/view/8004 SP - 268-271 AB - <p>Objective: The present study was undertaken to investigate the thrombolytic activity of Punica granatum fruit and peel extract.<br />Methods: Phytoconstituents and free radical scavenging activity of P. granatum extract were performed. Ethanolic and aqueous extract of fruit and<br />peel of the plant was used for in vitro clot lysis activity using streptokinase (SK) as standard. In a series of pre-weighed eppendorf tubes, equal volume<br />of venous blood was collected and serum was removed and the tubes were weighed. The tubes containing clot were incubated with fruit, peel extract<br />(100 ug/ml), and SK individually at 37°C for 60 minutes. After incubation, fluid obtained was removed, and tubes were again weighed to observe the<br />difference in weight after clot disruption. The percent of clot lysis was determined.<br />Results: Free radical scavenging activity of the extracts was increased in a dose-dependent manner. Of the extracts, ethanolic extract showed<br />maximum activity as compared with aqueous extract, whereas the ascorbic acid exerted maximum activity as compared with the extracts. Maximum<br />thrombolytic activity (59%) was observed with ethanolic extract of peel of the fruit.<br />Conclusions: The present study concluded that the aqueous and ethanolic extracts of P. granatum fruit and peel possess thrombolytic activity.<br />Keywords: Punica granatum, Antithrombolytic activity, Antibacterial, Antioxidant, Phytochemicals.</p> ER -