PREPARATION OF FERROUS SULFATE MICROCAPSULES AS A SUSTAINED RELEASE DOSAGE FORMS
Objective: The main purpose of this study was to optimize the different methods for the preparation for the preparation sustained release microencapsulated ferrous sulfate as a solid dosage form.
Methods: Ferrous sulfate was prepared as microcapsules using three microencapsulation methods. Complex coacervation, aqueous colloidal polymer dispersions, and solvent removal methods were used to prepare various formulas with different coating agents (acacia, gelatin, sodium alginate and ethylcellulose). The formation and texture characteristics, entrapment efficiency, release profiles, particle size and storage stability of ferrous microcapsules were evaluated in this study.
Results: The encapsulation efficiency and hardening varied considerably among these three preparation methods. Encapsulation of ferrous sulfate by complex coacervation with a coating agent (gelatin and acacia) showed problems in hardening and poor encapsulation efficiency. However, ferrous sulfate when coated by sodium alginate at 1:1 (coat: core) ratio using aqueous colloidal polymer dispersion method showed acceptable encapsulation efficiency (67%Â±0.1). Moreover, ferrous sulfate/sodium alginate microcapsules hardened successively when dropping into CaCl2 solution (2% w/v). A same hardening features and values of encapsulation efficiency (68 %Â±0. 6) Were obtained by solvent removal methods. Especially, after tween 80 and carboxyl methyl cellulose were added to the aqueous phase in the process of coating with ethylcellulose. However, sustained release microcapsules were produced by aqueous colloidal polymer dispersion method. The sustained-release sodium alginate/ferrous sulfate was stable for 30 d in both refrigeration and room temperature.
Conclusion: The aqueous polymer dispersion gave sustain release microcapsules which were uniform, hard and stable during storage at both room temperature and refrigeration.
Keywords: Ferrous sulfate, Microcapsules, Complex coacervation, Aqueous colloidal, Polymer dispersion, Solvent removal
2. Kuang SS, Oliveira JC, Crean AM. Microencapsulation as a tool for incorporating bioactive ingredients into food. Crit Rev Food Sci Nutr 2010;10:951â€“68.
3. Lim C, Kiesius, P H, Li H, Robinson EH. The interaction between dietary levels of iron and vitamin C on growth hematology. J Aquacult 2000;185:313-27.
4. Boccio JR, Caro RA, lysionek AE. Microencapsulated ferrous sulfate to fortify cow milk: absorption and distribution in mice. J Nutr Sci Vitaminol 1998;44:381-9.
5. Bhattacharyya A, Argillier JF. Microencapsulation by complex coacervation: effect of cationic surfactants. J Surf Sci Technol 2005;21:161-8.
6. Thangaraj S, Seethalakshmi M. Microencapsulation of vitamin C through an extrusion process. Int J Adv Res Biol Sci 2014;7:16â€“21.
7. Vuaridel E, Orsolini P. One-step dispersion method for the microencapsulation of water soluble substances. Eur Pat Appl 1999;10:683.
8. Kosaraju SL, Tran C, Lawrence A. Liposomal delivery systems for encapsulation of ferrous sulfate: preparation and characterization. J Liposome Res 2006;16:347-58.
9. Singh MN, Hemant KS, Ram M, Shivakumar HG. Microencapsulation a promising technique for controlled drug delivery. Res Pharm Sci 2010;5:65-77.
10. Sakarkar DM, Dorle AK, Mahajan NM, Sudke SG. The design of sustained release pellets of ferrous fumarate using cow ghee as hot-melt coating agent. Int J Pharm Invest 2013;33:151-6.
11. Azagheswari BK, Sathireddy P, Padma P. A review on microcapsules. Global J Pharmacol 2015;9:28-39.
12. Saravanan M. Preparation and characterization of nimesulide microcapsules by gelatin-pectin complex coacervation. Indian Drugs 2002;39:368â€“72.
13. McMullen JN, Newton DW, Becker H. Pectinâ€“complex gelatin coacervates: determinants of microglobulin size, morphology, and recovery as water dispersible powders. J Pharm Sci 1982;71:628â€“33.
14. Saravanan M, Rao KP. Pectinâ€“gelatin and alginateâ€“complex gelatin coacervation for controlled drug delivery: influence of anionic polysaccharides and drugs being encapsulated on physicochemical properties of microcapsules. Carbohydr Polym 2010;80:808-16.
15. Bodmeier R, Wang J. Microencapsulation of drugs with aqueous colloidal polymer dispersions. J Pharm Sci 1993;82:191-4.
16. Hongkee S. Microencapsulation techniques using ethyl acetate as a dispersed solvent: effects of Its extraction rate on the characteristics of PLGA microspheres. J Controlled Release 1997:47:233-45.
17. Sergio F, Hans PM, Bruno G. Microencapsulation by solvent extraction/evaporation: reviewing the state of the art of microsphere preparation process technology. J Controlled Release 2005;102:313â€“32.
18. Park JH. Reservoir-type microcapsules prepared by the solvent exchange method: effect of formulation parameters on microencapsulation of lysozyme. Mol Pharmaceutics 2006;3:2.
19. Wasfy MO: Recent patents review in microencapsulation of pharmaceuticals using the emulsion solvent removal methods. Recent Pat Drug Delivery Formulation 2009;3:178-92.
20. Biju SS. Dual coated erodible microcapsules for modified release of diclofenac sodium. Eur J Pharm Biopharm 2004;58:61-7.