ASPERGILLUS FLAVUS MEDIATED SILVER NANOPARTICLES SYNTHESIS AND EVALUATION OF ITS ANTIMICROBIAL ACTIVITY AGAINST DIFFERENT HUMAN PATHOGENS
Objective: Here, we report the extracellular synthesis of silver nanoparticles (AgNPs) using the cell-free extract of fungal isolate Aspergillus flavus and evaluation its inhibitory activity against bacterial pathogens.
Methods: Synthesized AgNPs was characterized via high throughput instrumentation such as UVâ€“Visible spectrophotometer (UV-Vis) and Fourier transform infrared spectroscopy, High-resolution transmission electron microscopy (HRTEM), X-ray Diffraction (XRD) and Energy dispersive X-ray spectroscopy (EDAX).
Results: Formation of yellowish brown colour clearly indicates the synthesis of AgNPs which produces a SPR peak at 420 nm. Active protein metabolites present in the cell-free extract plays a crucial role in reduction and stabilization of AgNPs. It was clearly observed that synthesized AgNPs were faced-centered cubic crystalline in nature with the mean size of 22Â±11 nm. Further, synthesized AgNPs capped with protein moieties exhibits excellent inhibitory activity against tested bacterial pathogens.
Conclusion: In this study, we have isolated the fungal strain A. flavus from the infected larvae of D. eucharis from the soil. The active metabolites of isolated A. flavus have been successfully used as an eco-friendly reducing agent to generate AgNPs and synthesized particles can be potentially developed as a drug candidature for antimicrobial therapy.
Keywords: A. flavus, Silver nanoparticles, HRTEM, Proteins, Human pathogens
2. Devi LS, Joshi SR. Evaluation of the antimicrobial potency of silver nanoparticles biosynthesized by using an endophytic fungus, Cryptosporiopsis ericae PS4. J Microbiol 2014;52:667-74.
3. Sathishkumar G, Bharti R, Jha PK, Selvakumar M, Dey G, Jha R, et al. Dietary flavone chrysin (5, 7-dihydroxyflavone ChR) functionalized highly-stable metal nanoformulations for improved anticancer applications. RSC Adv 2015;5:89869-78.
4. Narayanan KB, Sakthivel N. Biological synthesis of metal nanoparticles by microbes. Adv Colloid Interface Sci 2010;156:1â€“13.
5. Rahul BS, Satish VP, Chandrashekhar DP, Bipinchandra KS. The larvicidal potential of silver nanoparticles synthesized using fungus Cochliobolus lunatus against Aedes aegypti (Linnaeus, 1762) and Anopheles stephensi Liston (Diptera; Culicidae). Parasitol Res 2011;109:823â€“31.
6. Ahmad A, Mukherjee P, Senapati S, Mandal D, Khan MI, Kumar R, et al. Extracellular biosynthesis of AgNPs using the fungus Fusarium oxysporum. Colloids Surf B 2003;28:313â€“8.
7. Bhainsa KC, Dâ€™Souza SK. Extracellular biosynthesis of AgNPs using the fungus Aspergillus fumigates. Colloids Surf B 2006;47:160â€“4.
8. Gade AK, Bonde P, Ingle AP, Marcato PD, Duran N, Rai MK. Exploitation of Aspergillus niger for the synthesis of AgNPs. J Biobased Mater Bioenergy 2008;2:243-7.
9. Verma VC, Kharwar RN, Gange AC. Biosynthesis of antimicrobial AgNPs by the endophytic fungus Aspergillus clavatus. Nanomedicine 2010;5:33â€“40.
10. Qian Y, Yu H, He D, Yang H, Wang W, Wan X, et al. Biosynthesis of AgNPs by the endophytic fungus Epicoccum nigrum and their activity against pathogenic fungi. Bioprocess Biosyst Eng 2013;36:1613â€“9.
11. Musarrat J, Dwivedi S, Singh BJ, Al-Khedhairy AA, Azam A, Naqvi A. Production of antimicrobial AgNPs in water extracts of the fungus Amylomyces rouxii strain KSU-09. Bioresour Technol 2010;101:8772â€“6.
12. Sundararajan B, Mahendran G, Thamaraiselvi R, Kumari BDR. Biological activities of synthesized silver nanoparticles from Cardiospermum halicacabum L. Bull Mater Sci 2016;39:423â€“31.
13. Prabukumar S, Rajkuberan C, Ravindran K, Sivaramakrishnan S. Isolation and characterization of endophytic fungi from medicinal plant Crescentia cujete l. and their antibacterial, antioxidant and anticancer properties. Int J Pharm Pharm Sci 2015;7:316-21.
14. Pradeepa M, Kalidas V, Geetha N. Qualitative and quantitative phytochemical analysis and bactericidal activity of Pelargonium graveolens lâ€™her. Int J Appl Pharm 2016;8:7-11.
15. Chen JC, Lin ZH, Ma XX. Evidence of the production of silver nanoparticles via pretreatment of Phoma sp. 3.2883 with silver nitrate. Lett Appl Microbiol 2003;37:105â€“8.
16. Mohanpuria P, Nisha K, Rana NK, Yadav SK. Biosynthesis of nanoparticles: technological concepts and future applications. J Nanopart Res 2008;10:507â€“17.
17. Song JY, Kim BS. Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 2009;32:79â€“84.
18. Shrivastava S, Dash D. Label-free colorimetric estimation of proteins using nanoparticles of silver. Nano-Micro Lett 2010;2:164-8.
19. MubarakAli D, Thajuddin N, Jeganathan K, Gunasekaran M. Plant extract mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens. Colloids Surf B 2011;85:360â€“5.
20. Rajkuberan C, Prabukumar S, Sathishkumar G, Wilson A, Ravindran K, Sivaramakrishnan S. Facile synthesis of silver nanoparticles using Euphorbia anti quorum L. latex extract and evaluation of their biomedical perspectives as anticancer agents. J Saudi Chem Soc 2016. Doi:10.1016/j.jscs.2016.01.002. [Article in Press]
21. Panacek A, Kvitek L, Prucek R, Kolar M, Vecerova R, Pizurova N, et al. Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. J Phys Chem B 2006;110:16248â€“53.
22. Sathishkumar G, Gobinath C, Karpagam K, Hemamalini V, Premkumar K, Sivaramakrishnan S. Phyto-synthesis of nanoscale silver particles using Morinda citrifolia L. and its inhibitory activity against human pathogens. Colloids Surf B 2012;95:235-40.
23. Morones J, Elechiguerra J, Camacho A, Holt K, Kouri J, Ramirez J, et al. The bactericidal effect of silver nanoparticles. Nanotechnology 2005;16:2346-53.