Manyasree D, Kiran Mayi Peddi, Ravikumar R


Objective: In the present study copper oxide (CuO) nanoparticles were synthesized and characterized. The antibacterial activity of CuO nanoparticles was carried out against Escherichia coli, Proteus vulgaris, Staphylococcus aureus and Streptococcus mutans.

Methods: The synthesis was carried out by coprecipitation method using copper sulfate and sodium hydroxide as precursors. The synthesized copper oxide nanoparticles were characterized by using X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FT-IR), UV-vis spectroscopy and scanning electron microscope (SEM) with Energy Dispersive X-ray Analysis (EDX) techniques. Besides, this study determines the antibacterial activity and minimum inhibitory concentration (MIC) of CuO nanoparticles against gram-positive (Staphylococcus aureus and Streptococcus mutans) and gram-negative (E. coli and Proteus vulgaris) bacteria.

Results: The average crystallite size of CuO nanoparticles was found to be 19 nm by X-ray diffraction. FT-IR spectrum exhibited vibrational modes at 432 cm-1, 511 cm-1 and 611 cm-1were assigned for Cu-O stretching vibration. According to UV-Vis spectrum, two bands were observed at 402 nm and 422 nm. ED’s spectrum shows only elemental copper (Cu) and oxide (O) and no other elemental impurity was observed. The antimicrobial assay revealed that Proteus vulgaris showed a maximum zone of inhibition (37 mm) at 50 mg/ml concentration of CuO nanoparticles.

Conclusion: In conclusion, copper oxide is a good antibacterial agent against both gram positive and gram-negative organisms.


CuO nanoparticles, XRD, FTIR, SEM, EDS, Antibacterial activity

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Eustis MA, El-Sayed. Why gold nanoparticles are more precious than pretty gold: noble metal surface plasmon resonance and its enhancement of the radiative and nonradiative properties nanocrystals of different shapes. Chem Soc Rev 2006;35:209-17.

Samia ACS, Dayal S, Burda C. Quantum Dot-Based energy transfer: Perspectives potential for applications in photo dynamic therapy. J Photochem Photobiol 2006;82:617-25.

Gutierrez FM, Olive PL, Banuelos A, Orrantia E, Nino N, Sanchez EM, et al. A study on the antibacterial activity of Zno nanoparticles. J Nanomed 2010;6:681-8.

Guajardo-Pachecoa MJ, Morales-Sanchz JE, González-Hernándezc J, Ruiz F. Synthesis of copper nanoparticles using soybeans as a chelant agent. Mater Lett 2010;64:1361-4.

Singh J, Kaur G, Rawat M. A brief review on synthesis and characterization of copper oxide nanoparticles and its applications. J Bioelectron Nanotechnol 2016;1:1-9.

Sawsan Dagher, Yousef Haik, Ahmad I, Ayesh, Nacir Tit. Synthesis and optical properties of colloidal CuO nanoparticles. J Luminesce 2014;151:149-54.

Bouazizi N, Bargougui R, Oueslati A, Benslama R. Effect of synthesis time on structural, optical and electrical properties of CuO nanoparticles synthesized by reflux condensation method. Adv Mat Lett 2015;6:158.

Rohini Priya K, Suganthi KS, Rajan KS. Transport properties of ultra low concentration CuO water nanofluids containing nonspherical nanoparticles. Int J Heat Mass Transfer 2012;55:4734-43.

Hosseinpour M, Ahmadi SJ, Mousavand T, Outokesh MJ. Production of granulated-copper oxide nanoparticles for catalytic application. J Mat Res 2010;25:2025-34.

Henam Sylvia Devi, Thiyam David Singh. Synthesis of copper oxide nanoparticles by a novel method and its application in the degradation of methyl orange. Adv Electr Electric Eng 2014;4:83-8.

Hong L, Liu AL, Li GW, Chen W, Lin XH. Chemiluminescent cholesterol sensor based on Peroxidase like activity of cupric oxide nanoparticles. Biosens Bioelectron 2013;43:1-5.

Li XM, Wang L, Fan YB, Feng QL, Cui FZ. Biocompatibility and toxicity of nanoparticles and nanotubes. J Nanomater 2012;19.

Seil JT, Webster TJ. Antimicrobial applications of nanotechnology: methods and literature. Int J Nanomed 2012;7:2767-81.

Sengupta S, Chattopadhyay MK, Grossart HP. The multifaceted roles of antibiotics and antibiotic resistance in nature. Front Microbiol 2013;4:47-60.

Wright GD. Something new: revisiting natural products in antibiotic drug discovery. Can J Microbiol 2014;60:147-54.

Viswanathan VK. Off–label abuse of antibiotics by bacteria. Gut Microbes 2014;5:3-4.

Michael CA, Dominey-Howes D, Labbate M. The antimicrobial resistance crisis: causes, consequences, and management. Front Public Health 2014;2:145.

Ravi Chandrika K, Kiranmayi P, Ravikumar RVSSN. Synthesis, characterization and antibacterial activity of ZnO nanoparticles. Asian J Pharm Clin Res 2012;5:97-9.

Qi L, Xu Z, Jiang X, Hu C, Zou X. Preparation and antibacterial activity of chitosan nanoparticles. Carbohydr Res 2004;339:2693-700.

Ruparelia JP, Arup KC, Siddhartha P, Duttagupta, Suparna M. Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomater 2008;4:707-16.

Vijayalakshmi R, Rajendran V. Synthesis and characterization of nano-TiO2 via different methods. Arch Appl Sci Res 2012;4:1183-90.

Dobrucka R, Długaszewska J. Biosynthesis and antibacterial activity of Zno nanoparticles usingTrifolium pratense flower extract. Saudi J Biol Sci 2016;23:517-23.

Gopalakrishnan K, Ramesh C, Ragunathan V, Thamilselvan M. Antibacterial activity of copperoxide nanoparticles on E. coli synthesized from Tridaxprocumbensleaf extract and surface coating with polyaniline. Digest J Nanomater Biostructures 2012;7:833-9.

Stoimenov PK, Klinger RL, Marchin GL, Klabunde KJ. Metal oxide nanoparticles as bactericidal agents. Langmuir 2002;18:6679-86.

Ren G, Hu D, Cheng EWC, Vargas-Reus MA, Reip P, Allaker RP. Characterization of copper oxide nanoparticles for antimicrobial applications. Int J Antimicrob Agents 2009;33:587-90.

Koch AL, Woeste SW. The elasticity of the sacculus of Escherichia coli. J Bacteriol 1992;174:4811-9.

Elizabath Antony, Mythili Sathiavelu, Sathiavelu Arunachalam. Synthesis of silver nanoparticles from the medicinal plant bauhinia acuminata and biophytum sensitivum–a comparative study of its biological activities with plant extract. Int J Appl Pharm 2017;9:22-9.

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CuO nanoparticles, XRD, FTIR, SEM, EDS, Antibacterial activity





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International Journal of Applied Pharmaceutics
Vol 9, Issue 6 (Oct-Nov), 2017 Page: 71-74

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Authors & Affiliations

Manyasree D
Dept of Biochemistry, Acharya Nagarjuna University, NH16, Nagarjuna Nagar, Guntur, Andhra Pradesh 522510

Kiran Mayi Peddi
Dept of biochemistry, Acharya Nagarjuna University Nagarjuna University, NH16, Nagarjuna Nagar, Guntur, Andhra Pradesh 522510

Ravikumar R
Dept of Physics, acharya Nagarjuna University, NH16, Nagarjuna Nagar, Guntur, Andhra Pradesh 522510


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