CuO NANOPARTICLES: SYNTHESIS, CHARACTERIZATION AND THEIR BACTERICIDAL EFFICACY


Manyasree D, Kiran Mayi Peddi, Ravikumar R

Abstract


Objective: In the present study copper oxide (CuO) nanoparticles were synthesized and characterized. The antibacterial activity of CuO nanoparticles was carried out against Escherichia coli, Proteus vulgaris, Staphylococcus aureus and Streptococcus mutans.

Methods: The synthesis was carried out by coprecipitation method using copper sulfate and sodium hydroxide as precursors. The synthesized copper oxide nanoparticles were characterized by using X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FT-IR), UV-vis spectroscopy and scanning electron microscope (SEM) with Energy Dispersive X-ray Analysis (EDX) techniques. Besides, this study determines the antibacterial activity and minimum inhibitory concentration (MIC) of CuO nanoparticles against gram-positive (Staphylococcus aureus and Streptococcus mutans) and gram-negative (E. coli and Proteus vulgaris) bacteria.

Results: The average crystallite size of CuO nanoparticles was found to be 19 nm by X-ray diffraction. FT-IR spectrum exhibited vibrational modes at 432 cm-1, 511 cm-1 and 611 cm-1were assigned for Cu-O stretching vibration. According to UV-Vis spectrum, two bands were observed at 402 nm and 422 nm. ED’s spectrum shows only elemental copper (Cu) and oxide (O) and no other elemental impurity was observed. The antimicrobial assay revealed that Proteus vulgaris showed a maximum zone of inhibition (37 mm) at 50 mg/ml concentration of CuO nanoparticles.

Conclusion: In conclusion, copper oxide is a good antibacterial agent against both gram positive and gram-negative organisms.


Keywords


CuO nanoparticles, XRD, FTIR, SEM, EDS, Antibacterial activity

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About this article

Title

CuO NANOPARTICLES: SYNTHESIS, CHARACTERIZATION AND THEIR BACTERICIDAL EFFICACY

Keywords

CuO nanoparticles, XRD, FTIR, SEM, EDS, Antibacterial activity

DOI

10.22159/ijap.2017v9i6.71757

Date

08-11-2017

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 9, Issue 6 (Oct-Nov), 2017 Page: 71-74

Online ISSN

0975-7058

Statistics

40 Views | 6 Downloads

Authors & Affiliations

Manyasree D
Dept of Biochemistry, Acharya Nagarjuna University, NH16, Nagarjuna Nagar, Guntur, Andhra Pradesh 522510

Kiran Mayi Peddi
Dept of biochemistry, Acharya Nagarjuna University Nagarjuna University, NH16, Nagarjuna Nagar, Guntur, Andhra Pradesh 522510
India

Ravikumar R
Dept of Physics, acharya Nagarjuna University, NH16, Nagarjuna Nagar, Guntur, Andhra Pradesh 522510


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