• Carolin Parlina Department of Orthodontics, Faculty of Dentistry, Universitas Indonesia
  • Erni H Purwaningsih Department of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia
  • Ahmad Aulia Jusuf Department of Medical Histology, Faculty of Medicine, Universitas Indonesia
  • Retno Widayati Department of Orthodontics, Faculty of Dentistry, Universitas Indonesia


Objective: This study aimed to show the impact of the ZOL in VCO gel (Ge-ZOL) on the extent of osteoclasts apoptosis.

Methods: The study used 27 Sprague-Dawley rats which were divided into three groups: Nine rats in the experimental group were given 40 µg of Ge-ZOL, nine rats in the control group were given VCO emulsion gel without ZOL (Ge-), and nine rats in the normal group were not given any treatment. The gel was applied to the buccal mucosa using a cotton bud for 2 min at hour of 0, 4, and 8 on days 0, 1, 2, 3, and 4. The rats were sacrificed on days 1, 3, and 5, and then, evaluated by immunohistochemical caspase-3 staining.

Result: The number of apoptotic osteoclast cells in the experimental group was significantly higher than in the control and normal groups (p<0.05). The number of apoptotic osteoclast cells in the experimental group on the day 1 was significantly higher than on the days 3 and 5 (p<0.001).

Conclusion: The application of Ge-ZOL to the buccal mucosa proven to improve the number of apoptotic osteoclast cells in the experimental group on the day 1, and this number was higher than on the days 3 and 5.
Keywords: Zoledronate bisphosphonate, Virgin coconut oil, Caspase-3, Osteoclast apoptosis.


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How to Cite
Parlina, C., Purwaningsih, E. H., Jusuf, A. A., & Widayati, R. (2018). IMPACT OF ZOLEDRONATE BISPHOSPHONATE GEL IN VIRGIN COCONUT OIL ON THE INCREASE OF OSTEOCLAST APOPTOSIS. International Journal of Applied Pharmaceutics, 9, 24-27.
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