ROSELLE CALYX ETHANOL EXTRACT STIMULATES ORAL MUCOSAL WOUND HEALING
Objective: This study aim to analyze the effect of the roselle calyx ethanol extract on oral mucosal wound healing.
Methods: Male Sprague Dawley rats (3Â month, 150Â g) were housed under standard temperature conditions (20â€“22Â°C). Punch biopsy wounds were inflicted on the inferior labial mucosal membrane of rats. All treatments (normal saline; 0.1% triamcinolone acetonide; and 3.75%, 7.5%, and 15% roselle calyx ethanol extract) were applied 2Â times daily. Wounded areas were observed after 8Â days of treatment. The specimen of the wounded area was prepared and microscopic slides were analyzed under a light microscope.
Results: The histological features of the groups treated with TA and 7.5% roselle calyx ethanol extract showed a similar formation of the epidermis layer and no lymphocytic infiltration of the lamina propria.Conclusion: The application of 7.5% roselle calyx ethanol extract could stimulate oral mucosal wound healing.
2. Jain SJ, Jain N, Tiwari A, Balekar N, Jain DK. Simple evaluation of wound healing activity of polyhedral formulation of roots of Ageratum conyzoides Linn. Asian J Res Chem 2009;2:135-8.
3. Erhunmwunse MO, Odiase DE. Histological effects of chronic adminstration of crude aqueous extract of Hibiscus sabdariffa on the colon of Wistar rats. Int J Adv Pharm Sci Technol 2014;2:21-6.
4. Builders PF, Kabele-Toge B, Builders M, Chindo BA, Anwunobi PA, Isimi YC. Wound healing potential of formulated extract from hibiscus sabdariffa calyx. Indian J Pharm Sci 2013;75:45-52.
5. Sarr M, Ngom S, Kane MO, Wele A, Diop D, Sarr B, et al. In vitro vasorelaxation mechanisms of bioactive compounds extracted from Hibiscus sabdariffa on rat thoracic aorta. Nutr Metab (Lond)2009;6:45.
6. Kim YS, Cho IH, Jeong MJ, Jeong SJ, Nah SY, Cho YS, et al. Therapeutic effect of total ginseng saponin on skin wound healing. J Ginseng Res 2011;35:360-7.
8. Eltayeib AA, Hamade H. Phytochemical and chemical composition of water extract of Hibiscus sabdariffa (Red karkade calyces) in North Kordofan Sate-Sudan. Int J Adv Res Chem Sci 2014;1:10-3.
9. Alqasoumi S, Al-Dosari M, Al-Sohaibani M, Al-Howiriny T, Al-Yahya M, Rafatullah S. Gastric ulcer protective activity of Hibiscus sabdariffa: An experimental, biochemical and histology study. Clin Exp Med J 2010;4:115-27.
10. Patel S. Hibiscus sabdariffa: An ideal yet under-exploited candidate for nutraceutical applications. Biomed Prev Nutr 2014;4:23-7.
11. Ochani PC, Dâ€™Mello P. Antioxidant and antihyperlipidemic activity of Hibiscus sabdariffa Linn. leaves and calyces extracts in rats. Indian J Exp Biol 2009;47:276-82.
12. Gosain S, Ircchiaya R, Sharma PC, Thareja S, Kalra A, Deep A, et al. Hypolipidemic effect of ethanolic extract from leaves of Hibiscus sabdariffa Linn in hyperlipidemic rats. Acta Pol Pharm 2010;67:179-84.
13. Cid-Ortega S, Guerrero-Beltran JA. Roselle calyces (Hibiscus sabdariffa), an alternative to the food and beverages indsutries: A review. J Food Sci Technol 2015;52:6859-69.
14. Al-Budairy A, Fadil N, Al-Ameer MA. Histological study of effect of thyroxin and aqueous extract of karkade on liver in Swiss male mice. Mag Al-Kufa Univ Biol 2011;3:201-9.
15. Mohamed R, FernÃ¡ndez J, Pineda M, Aguilar M. Roselle (Hibiscus sabdariffa) seed oil is a rich source of gamma-tocopherol. J Food Sci 2007;72:S207-11.
16. Sukhitashvili N, Imnadze I, Tabaghua G, Gogilashvili Q, Amiranashvili I. Characterization of oral ulcer and pathological scar in nude mice model. Georgian Med News 2012;205:82-7.
17. Ali MK, Ashraf A, Biswas NN, Karmakar UK, Afroz S. Antinociceptive, anti-inflammatory and antidiarrheal activities of ethanolic calyx extract of Hibiscus sabdariffa Linn. (Malvaceae) in mice. Zhong Xi Yi Jie He Xue Bao 2011;9:626-31.
18. Begashaw B, Mishra B, Tsegaw A, Shewamene Z. Methanol leaves extract Hibiscus micranthus Linn exhibited antibacterial and wound healing activities. BMC Complement Altern Med 2017;17:337.
19. Fullerton M, Khatiwada J, Johnson JU, Davis S, Williams LL. Determination of antimicrobial activity of sorrel (Hibiscus sabdariffa) on Escherichia coli O157:H7 isolated from food, veterinary, and clinical samples. J Med Food 2011;14:950-6.
20. Abdul Hamid Z, Lin Lin WH, Abdalla BJ, Bee Yuen O, Latif ES, Mohamed J, et al. The role of Hibiscus sabdariffa L. (Roselle) in maintenance of ex vivo murine bone marrow-derived hematopoietic stem cells. Sci World J 2014;2014:258192.