THE OPTIMIZATION OF ELUTING CONDITION OF SOLID PHASE EXTRACTION METHOD FOR ï¡-MANGOSTIN PURIFICATION IN MANGOSTEEN PERICARP EXTRACT
Keywords:Nil, Eluent, Optimization, Solid phase extraction
Objective: To obtain the optimum condition of eluent selection for purification of Î±-mangostin in mangosteen pericarp extract with solid phase extraction (SPE) method.
Methods: The analysis was conducted by SPE cartridge octadecylsilane (ODS)-5, solvents used were methanol for conditioning, aqua bidestilated for washing and three different eluents for eluting: methanol, ethanol and ethyl acetate.
Results: Recovery value for each eluent were 32.4741% for methanol, 46.6130% for ethanol and 33.6383% for ethyl acetate. Comparison of alpha-mangostin level in the mangosteen pericarp extract for each eluent i.e. 14.4578% for methanol, 10.0598% for ethanol, and 14.2898 % for ethyl acetate while compared to the extract without SPE was 21.7934%.
Conclusion: Ethanol had the highest recovery value, compared to methanol and ethyl acetate.
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