• Shyla M. Haqq Department of Chemistry, SHUATS University, Allahabad, India
  • Himanshu Pandey Department of Pharmaceutical Sciences, SHUATS University, Allahabad, India
  • Manju Gerard ICAR- NAARM, Hyderabad
  • Amit Chattree Department of Chemistry, SHUATS University, Allahabad, India


Objective: In the present research work silver nanoparticles were synthesized using the flower extract of Chrysanthemum coronarium and their in-vitro antibacterial activity was evaluated against both the gram-positive S. aureus and gram-negative bacteria E. coli. The flower extract acted both as a reducing as well as a capping agent.

Methods: Silver nanoparticles were verified using various spectroanalytical techniques such as visible ultraviolet spectroscopy, zeta potential, fourier transform infrared spectroscopy and particle size analyser. The antibacterial activity was evaluated against both the gram-positive bacteria S. aureus and gram-negative bacteria E. coli using the agar well diffusion method.

Results: The silver nanoparticles synthesized were confirmed by the visual colour change. The ultraviolet, visible spectroscopy showed a surface plasmon resonance at 430 nm. Zetapotential was found to be around-15.6mV where the negative value indicated that the synthesized silver nanoparticles are stable. Fourier transform infrared spectroscopy showed the functional groups responsible for the stabilization of the nanoparticles. Particle size analyser showed that the size of the nanoparticles ranged from 5-50 nm. The antibacterial activity of the silver nanoparticles which was performed against S. aureus and E. coli showed good inhibition against both the bacteria. Better antibacterial activity was found for E. coli in comparison to S. aureus as the zone of inhibition for E. coli was found to be at 12 mm at 50ug/ml whereas the zone of inhibition against S. aureus was found to be at 10 mm.

Conclusion: The silver nanoparticles were successfully synthesized using a green approach and can be used as a potential resource for therapeutic purpose.

Keywords: Antibacterial activity, Silver nanoparticles, Chrysanthemum coronarium, flower extract, Gram positive and gram negative bacteria


1. Sadowski Z. Biosynthesis and application of silver and gold nanoparticles. Wrodaw. University Technol 2010;1:67-71.
2. Wang J, Wang Z. Synthesis of flower-like CuO nanostructures via a simple hydrolysis route. Mater Lett 2007;61:41-9.
3. Sharma KV, Yngard AR, Lin Y. Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 2009;145:83-96.
4. Ingale GA, Chaudhari NA. Biogenic synthesis of nanoparticles and potential applications. an eco-friendly approach. J Nanomed Nanotechnol 2013;4:1-7.
5. Ahmad N, Sharad B, Ali SS, Dutta R. Photofabrication of bio-induced silver nanoparticles for the biomedical applications. Int J Nanomed 2015;10:7019-30.
6. Bar-Eyal M, Sharon E, Spiegel Y. Nematicidal activity of Chrysanthemum coronarium. Eur J Plant Pathol 2006;114:427-33.
7. Alvarez Castellanos PP, Bishop CD, Pascual P, Maria J. Antifungal activity of the essential oil of flower heads of garland Chrysanthemum coronarium against agricultural pathogens. Phytochemistry 2001;57:99-102.
8. Lee DK, Park HK, Ha JT, Han SH, Yang SM. Isolation of pyrethrosin derivatives from the of Chrysanthemum coronarium L. Agric Chem Biotechnol 2003;46:76-9.
9. Yan H, Zhiyun UD, Jia QH, Zhikai T, Zheng Xi, Zhang K, et al. Green synthesis of silver nanoparticles by Chrysanthemum morifolium Ramat extract and their application in the clinical ultrasound gel. Int J Nanomed 2013;8:1809-15.
10. Anthony E, Sathiavelu M, Arunachalam S. Synthesis of silver nanoparticles from the medicinal plant Bauhinia acuminata and Biophytum sensitivum-a compartitive study of its biological activities with plant extract. Int J Appl Pharm 2017;9:22-9.
11. Menon S, Agarwal H, Kumar RS, Venkat kumar S. Green synthesis of silver nanoparticles using medicinal plant Acalypha indica leaf extracts and its application as an antioxidant and antimicrobial agent against foodborne pathogens. Int J Appl Pharm 2017;9:42-50.
12. Bhumi G, Rao G, Savithramma N. Green synthesis of silver nanoparticles from the leaf extract of Adhatoda vasica nees and assessment of its antibacterial activity. Asian J Pharm Clin Res 2015;8:42-50.
13. Jiang H, Manolache S, Wong LCA, Denes SF. Plasma–enhanced deposition of silver nanoparticles on to polymer and metal surfaces for the generation of antimicrobial characteristics. J Appl Polym Sci 2004;93:1411-22.
14. Patel S, Sivaraj R, Rajiv P, Venckatesh R, Seenivasan R. Green synthesis of silver nanoparticles from the leaf extract of Aegle marmelos and evaluation of its antibacterial activity. Int J Pharm Pharm Sci 2015;7:169-73.
15. Fatimah Is, Indrani N. Silver nanoparticles synthesized using lanta camara flower extract by reflux, microwave and ultrasound methods. Chem J Moldova 2018;13:1857-27.
16. Ali AZ, Yahya R, Sekaran DS, Puteh R. Green synthesis of silver nanoparticles using the apple extract and its antibacterial properties. Adv Mater Sci Eng 2016;1-6. 10.1155/2016/4102196
17. Ibrahim HM, Ibrahiem AA, Dalloul TR. Biosynthesis of silver nanoparticles using pomegranate juice extract and its antibacterial activity. Int J Appl Sci Biotechnol 2016;4:254-8.
18. Chandrappa CP, Chandrasekar N, Govinappa M, Shanbhag Chaitra, Singh KU, Masarghal J. Antibacterial activity of synthesized silver nanoparticles by simaroubaglauca against pathogenic bacteria. Int J Curr Pharm Res 2017;9:20-2.
19. Shrivastava S, Bera T, Roy A, Singh G, Ramachandrarao P, Dash D. Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology 2007;18:103-12.
20. Rinna A, Magdolenova Z, Hudecova A, Kruzewski M, Refsnes M, Dusinska M. Mutagenesis 2015;30:59-66.
21. Li RW, Xie BX, Shi SQ, Zeng YH, Ou-yang SY, Chen BY. Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Appl Microbiol Biotechnol 2010;85:1115-22.
22. Mata R, Bhaskaran A, Sadras RS. Green-synthesized gold nanoparticles from Plumeria alba flower extract to aument catalytic degradation of organic dyes and inhibit bacterial growth. Particuology 2015;24:78-86.
177 Views | 135 Downloads
How to Cite
Haqq, S., Pandey, H., Gerard, M., & Chattree, A. (2018). BIO-FABRICATION OF SILVER NANOPARTICLES USING CHRYSANTHEMUM CORONARIUM FLOWER EXTRACT AND IT’S IN VITRO ANTIBACTERIAL ACTIVITY. International Journal of Applied Pharmaceutics, 10(5), 209-213.
Original Article(s)