ANTIBACTERIAL AND ANTICANCER POTENTIAL OF SILVER NANOPARTICLES SYNTHESIZED USING GALLIC ACID IN BENTONITE/STARCH BIO-NANOCOMPOSITES


Anupama Thapliyal, Amrish Chandra

Abstract


Objective: To optimize and synthesize eco-friendly and low-cost silver nanoparticles (AgNPs) by using gallic acid (GA) reducing agent in bentonite/starch bio-nanocomposites (BNCs) for oral use and to evaluate its antibacterial and anticancer efficacy.

Methods: An artificial neural network (ANN) model was employed for the optimization and evaluate the effect of the formulation variables on the entrapment efficiency (EE) of AgNPs. The synthesized AgNPs in BNCs were characterized using UV-vis spectroscopy, energy dispersive X-ray spectroscopy (EDXA), dynamic light scattering (DLS), scanning electron microscopy (SEM), zeta potential and fourier transform infrared spectroscopy (FTIR). Elemental ion analysis was carried out using inductively coupled plasma mass spectrometry (ICP-MS). Drug release study was carried out. The antimicrobial efficacy determined by agar well diffusion method. In vitro anticancer efficacy of AgNPs in breast cancer cell line (MCF-7) by MTT assay was performed.

Results: The formation of AgNPs was confirmed by UV-vis absorbance peak shown at 412 nm. XRD spectrum has indicated the face-centered cubic structure of the synthesized AgNPs. SEM and DLS measurements showed spherical nanoparticles with a mean size of 68.06±0.2 nm. The negative surface zeta potential with-32±0.25 mV has indicated colloidal stability of nanoparticles. FTIR spectra confirmed no interaction observed between drug and excipients. AgNPs showed significant EE with 80±0.25%. The synthesized AgNPs in BNCs is a potential candidate for inhibiting the growth of pathogenic bacteria and showed significant cytotoxicity against MCF-7 cancer cell line with IC50 of 160±0.014μg/ml.

Conclusion: The present research confirms that the green synthesized AgNPs in BNCs can be a promising antibacterial and anticancer agent regarding stability, low cost and easy preparation.


Keywords


ANN model, Gallic acid, Silver nanoparticles, Bio-nanocomposites, Entrapment efficiency, Release kinetics, Antibacterial, Cytotoxicity

| PDF | HTML |

References


Bhatte KD, Tambade PJ, Dhake KP, Bhanage BM. Silver nanoparticles as an efficient, heterogeneous and recyclable catalyst for synthesis of β-enaminones. Catalysis Commun 2010;11:1233–7.

Narayanan KB, Sakthivel N. Biological synthesis of metal nanoparticles by microbes. Adv Colloid Interface Sci 2010; 156:1–13.

Carotenuto G, Pepe GP, Nicolais L. Preparation and characterization of nano-sized Ag/PVP composites for optical applications. Eur Phy J B 2000;16:11–7.

Stathatos E, Lianos P, Falaras P, Siokou A. Photocatalytically deposited silver nanoparticles on mesoporous TiO2 films. Langmuir 2000;16:2398–400.

Kyriacou SV, Brownlow WJ, Xu XHN. Using nanoparticle optics assay for direct observation of the function of antimicrobial agents in single live bacterial cells†. Biochem 2004;43:140–7.

Feng X, Ma H, Huang S, Pan W, Zhang X, Tian F, et al. Aqueous−organic phase-transfer of highly stable gold, silver, and platinum nanoparticles and a new route for the fabrication of gold nanofilms at the Oil/Water interface and on solid supports. J Physical Chemis B 2006;110:12311–7.

Choi S, Kim KS, Yeon SH, Cha JH, Lee H, Kim CJ, et al. Fabrication of silver nanoparticles via self-regulated reduction by 1-(2-hydroxyethyl)-3-methylimidazolium tetrafluoroborate. Korean J Chem Eng 2007:24:856–9.

Mohanta YK, Nayak D, Biswas K, Singdevsachan SK, Abd Allah EF, Hashem A, et al. Silver nanoparticles synthesized using wild mushroom show potential antimicrobial activities against foodborne pathogens. Molecules 2018;23:655.

Zhang L, Yu JC, Yip HY, Li Q, Kwong KW, Xu AW, et al. Ambient light reduction strategy to synthesize silver nanoparticles and silver-coated TiO2 with Enhanced photocatalytic and bactericidal activities. Langmuir 2003;19:10372–80.

Matijevic E. Preparation and properties of uniform size colloids. Chem Mate 1993:5:412–26.

Nickel U, Castell A, Pöppl K, Schneider SA. Silver colloid produced by reduction with hydrazine as support for highly sensitive surface-enhanced raman spectroscopy. Langmuir 2000;16:9087–91.

Leopold N, Lendl BA. New method for fast preparation of highly surface-enhanced raman scattering (SERS) active silver colloids at room temperature by reduction of silver nitrate with hydroxylamine hydrochloride. J Phys Chem B 2003;107:5723–7.

Khanna PK, Subbarao VVVS. Nanosized silver powder via reduction of silver nitrate by sodium formaldehyde sulfoxylate in acidic pH medium. Mate Lett 2003;57:2242–5.

Sondi I, Goia DV, Matijević E. Preparation of highly concentrated stable dispersions of uniform silver nano-particles. J Coll Int Sci 2003;260:75–81.

Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, et al. The bactericidal effect of silver nanoparticles. Nanotechnol 2005;16:2346–53.

Baker C, Pradhan A, Pakstis L, Pochan DJ, Shah SI. Synthesis and antibacterial properties of silver nanoparticles. J. Nanosci Nanotechnol 2005;5:244–9.

Shameli K, Ahmad MB, Zargar M, Yunis WM, Ibrahim NA, Shabanzadeh P, et al. Synthesis and characterization of silver/ montmorillonite/chitosan bionanocomposites by chemical reduction method and their antibacterial activity. Int J Nanomed 2011;271-84.

Ahmad. Synthesis and characterization of silver/clay/chitosan bionanocomposites by uv-irradiation method. Am J Appl Sci 2009;6:2030–5.

Darder M, Aranda P, Ruiz-Hitzky E. Bionanocomposites: a new concept of ecological, bioinspired, and functional hybrid materials. Adv Materials 2007;19:1309–19.

Mallakpour S, Dinari M. Synthesis and properties of biodegradable poly (vinyl alcohol)/Organo-nanoclay bionanocomposites. J Polymers Environ 2012;20:732–40.

Ma X, Chang PR, Yang J, Yu J. Preparation and properties of glycerol plasticized-pea starch/zinc oxide-starch bionanocomposites. Carbohydrate Polymers 2009;75:472–8.

Gavade C, Shah S, Singh NL, Garg AB, Mittal R, Mukhopadhyay R. Synthesis of silver polymer nanocomposites and their antibacterial activity. AIP 2011;1349:168.

Shabanzadeh P, Yusof R, Shameli K. Artificial neural network for modeling the size of silver nanoparticles’ prepared in montmorillonite/starch bionanocomposites. J Industrial Eng Chem 2015;24:42–50.

Nazari AG, Mozafari M. Simulation of structural features on the mechanochemical synthesis of Al2O3–TiB2 nanocomposite by the optimized artificial neural network. Adv Powder Technol 2012;23:220–7.

Shabanzadeh P, Senu N, Shameli K, Tabar MM. Artificial intelligence in numerical modeling of silver nanoparticles prepared in montmorillonite interlayer space. J Chemistry 2013;1–8. http://dx.doi.org/10.1155/2013/305713.

Ozdemir U, Ozbay B, Veli S, Zor S. Modeling adsorption of sodium dodecyl benzene sulfonate (SDBS) onto polyaniline (PANI) by using multilinear regression and artificial neural networks. Chem Eng J 2011;178:183–90.

Abdul Rahman MB, Chaibakhsh N, Basri M, Salleh AB, Abdul Rahman RNZR. Application of artificial neural network for yield prediction of lipase-catalyzed synthesis of dioctyl adipate. Appl Biochem Biotechnol 2009;158:722–35.

Khayet M, Cojocaru C, Essalhi M. Artificial neural network modeling and response surface methodology of desalination by reverse osmosis. J Membrane Sci 2011;368:202–14.

Shabanzadeh P, Yusof R, Shameli K. Modeling of biosynthesized silver nanoparticles in Vitex negundo L. extract by the artificial neural network. RSC Adv 2015;5:87277–85.

Demuth H, Beale M. Neural network toolbox for use with matlab, The Math-Works, Inc, MA; 1998.

Awotwe Otoo D, Zidan AS, Rahman Z, Habib MJ. Evaluation of anticancer drug-loaded nanoparticle characteristics by nondestructive methodologies. AAPS Pharm Sci Tech 2012; 13:611–22.

Valodkar M, Nagar PS, Jadeja RN, Thounaojam MC, Devkar RV, Thakore S. Euphorbiaceae latex-induced green synthesis of non-cytotoxic metallic nanoparticle solutions: a rational approach to antimicrobial applications. Colloids Surf A 2011;384:337–44.

Kareem S, Akpan I, Ojo O. Antimicrobial activities of calotropisprocera on selected pathogenic microorganisms. Afr J Biomed Res 2010;11:33.

Uma Suganyaa KS, Govindarajua K, Ganesh Kumara D, Prabhu C, Arulvasu T, Stalin Dhasa V, et al. Anti-proliferative effect of biogenic gold nanoparticles against breast cancer cell lines (MDA-MB-231 and MCF-7). Appl Sur Sci 2016;371:415–24.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55–63.

El-Naggar NEA, Hussein MH, El-Sawah AA. Bio-fabrication of silver nanoparticles by phycocyanin, characterization, in vitro anticancer activity against breast cancer cell line and in vivo cytotoxicity. Sci Reports 2017;7:10844.

Theivasanthi T, Alagar M. Konjac biomolecules assisted–rod/spherical shaped lead nanopowder synthesized by an electrolytic process and its characterization studies. Nano Biomedicine Eng 2013;5:2.

Alemdar A, Gungor N, Ece OI, Atici O. The rheological properties and characterization of bentonite dispersions in the presence of non-ionic polymer PEG. J Materials Sci 2005;40:171–7.

Wang W, Chen Q, Jiang C, Yang D, Liu X, Xu S. One-step synthesis of biocompatible gold nanoparticles using gallic acid in the presence of poly-(N-vinyl-2-pyrrolidone). Colloids Surf A 2007;301:73–9.

Shameli K, Ahmad MB, ZW Yunis WZ, Rustaiyan A, Ibrahim NA, Mohsen Z, et al. Green synthesis of silver/montmorillonite/ chitosan bionanocomposites using the UV irradiation method and evaluation of antibacterial activity. Int J Nanomed 2010;5:875-7.

Elbaz NM, Ziko L, Siam R, Mamdouh W. Core-shell silver/polymeric nanoparticles-based combinatorial therapy against breast cancer in vitro. Sci Reports 2016;6. Doi:10.1038/srep30729

Krishnaraj C, Jagan EG, Rajasekar S, Selvakumar P, Kalaichelvan PT, Mohan N. Synthesis of silver nanoparticles using Acalyphaindica leaf extracts and its antibacterial activity against water borne pathogens. Colloids Surf B 2010;76:50–6.

Ofokansi KC, Adikwu MU, Okore VC. Preparation and evaluation of mucin-gelatin mucoadhesive microspheres for rectal delivery of ceftriaxone sodium. Drug Dev Indus Pharm 2007;33:691–700.

Kuksal A, Tiwary AK, Jain NK, Jain S. Formulation and in vitro, in vivo evaluation of extended-release matrix tablet of Zidovudine: Influence of a combination of hydrophilic and hydrophobic matrix formers. AAPS PharmSciTech 2006;7:1–9.

Guzman M, Dille J, Godet S. Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria. Nanomed: Nanotechnol Biol Med 2012;8:37–45.




About this article

Title

ANTIBACTERIAL AND ANTICANCER POTENTIAL OF SILVER NANOPARTICLES SYNTHESIZED USING GALLIC ACID IN BENTONITE/STARCH BIO-NANOCOMPOSITES

Keywords

ANN model, Gallic acid, Silver nanoparticles, Bio-nanocomposites, Entrapment efficiency, Release kinetics, Antibacterial, Cytotoxicity

DOI

10.22159/ijap.2018v10i5.27728

Date

08-09-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 10, Issue 5 (Sep-Oct), 2018 Page: 178-189

Online ISSN

0975-7058

Authors & Affiliations

Anupama Thapliyal
Amity Institute of Pharmacy, Amity University, Noida, India
India

Amrish Chandra
Amity Institute of Pharmacy, Amity University, Noida, India
India


Refbacks

  • There are currently no refbacks.