PROTECTIVE EFFICACY OF SILVER NANOPARTICLES SYNTHESIZED FROM SILYMARIN ON CISPLATIN INDUCED RENAL OXIDATIVE STRESS IN ALBINO RAT


Janakiraman M.

Abstract


Objectives: This present study was carried out to evaluate the protective efficacy of silver nanoparticles synthesized from silymarin on cisplatin induced renal oxidative stress in albino rat.

 

Methods: Silver nanoparticles of silymarin was characterized by particle size,UV-Visible,FTIR and XRD analysis. Albino rats were divided randomly into six groups of six animals each. Group I Normal rats were treated with oral dose of distilled water for 15 days. Group II rats were treated with single i.p.dose of cisplatin (16 mg/kg) on day 1. Group III rats were treated only with oral dose of silymarin (50 mg/kg/d) for 15 days. Group IV rats were treated only with oral dose of silver nanoparticles of silymarin (50 mg/kg/d) for 15 days. Group V rats were treated with oral dose of silymarin for 14 days after single i.p.dose of cisplatin on day 1.Group VI rats were treated with oral dose of silver nanoparticles of silymarin for 14 days after single i.p.dose of cisplatin on day 1. Collected blood samples and kidney tissue samples were used for biochemical, enzymatic antioxidant and histopathalogical studies in all groups.

 

Results: The formation of silver nanoparticles of silymarin were confirmed by particle size,UV-Visible analysis, crystalline nature was confirmed by X-ray diffraction analysis and active principles were confirmed by FTIR analysis.Biochemical results of silver nanoparticles of silymarin treated groups showed the significant (p˂0.05) decrease in the level of creatinine, urea and uric acid as compared to cisplatin induced rats and The enzymatic antioxidants of silver nanoparticles of silymarin treated groups showed the significant (p˂0.05) increase in the level of glutathione reductase and significant decrease in MDA level as compared to cisplatin induced rats. Additionally, histopathological results of silver nanoparticles of silymarin treated groups also confirmed that the ameliorative effect of silymarin nanaoparticle against cisplatin induced rats.

 

Conclusion: From this research work, we have concluded that silver nanoparticles of silymarin protected the kidney of albino rats from the adverse effects caused by cisplatin.


Keywords


Silymarin,Cisplatin,Silver Nanoparticles,Nephrotoxicity,Histopathology

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