DESIGN OF EXPERIMENTS MODEL FOR THE OPTIMIZATION OF SILK FIBROIN BASED NANOPARTICLES


Duy Toan Pham, Nuttawut Saelim, Waree Tiyaboonchai

Abstract


Objective: Silk fibroin based nanoparticles have been utilized extensively in biomedical fields. Amongst many preparation methods, desolvation is a favorable one. However, this method yields nanoparticles with unpredictable parameters. Thus, this investigation aimed to systematically study the effects of three independent variables including fibroin concentration (% w/v, X1), volume ratio between fibroin solution and ethanol (X2), formulation time (h, X3) on three main responses, particle size (nm, Y1), polydispersity index (Y2), zeta potential (mV, Y3).

Methods: Fibroin was extracted from degummed Bombyx mori silk. The fibroin calibration curve was constructed by UV-spectrophotometer at 276 nm. The nanoparticles were prepared using the desolvation method of aqueous fibroin solution in ethanol. Design Expert® software was used to design the model. The mean particle size, polydispersity index and zeta potential were determined using ZetaPALS®analyzer.

Results: By using D-optimal design with the quadratic model, the results showed that all X1, X2, and X3 variables had significant impacts on the fibroin nanoparticles characteristics Y1, Y2, and Y3. The generated model was also validated and demonstrated to be solid and reliable. The obtained optimal nanoparticles possessed Y1 of 238.1 nm, Y2 of 0.12, and Y3of-21.78 mV, which were in agreement with the predicted values, 224.8 nm, 0.13 and-19.31 mV, respectively. The optimal actual and theoretical particle characteristics were correlated with a desirable value of R2 = 0.8770.

Conclusion: The D-optimal design proved its effectiveness in the prediction and optimization of fibroin nanoparticle properties.


Keywords


design of experiments; DOE; D-optimal; optimization; validation; fibroin; nanoparticles

| PDF | HTML |

References


Sahoo SK, Labhasetwar V. Nanotech approaches to drug delivery and imaging. Drug Discovery Today 2003;8:1112-20.

Wilczewska AZ, Niemirowicz K, Markiewicz KH, Car H. Nanoparticles as drug delivery systems. Pharmacol Rep 2012;64:1020-37.

Duarah S, Pujari K, Durai RD, Narayanan VHB. Nanotechnology-based cosmeceuticals: a review. Int J Appl Pharm 2016;8:8-12.

Banik BL, Fattahi P, Brown JL. Polymeric nanoparticles: the future of nanomedicine. Wiley Interdiscip Rev Nanomed Nanobiotechnol 2015;8:271-99.

Mottaghitalab F, Farokhi M, Shokrgozar MA, Atyabi F, Hosseinkhani H. Silk fibroin nanoparticle as a novel drug delivery system. J Controlled Release 2015;206:161-76.

Volkov V, Ferreira AV, Cavaco-Paulo A. On the routines of wild-type silk fibroin processing toward silk-inspired materials: a review. Macromol Mater Eng 2015;300:1199-216.

Zhao Z, Li Y, Xie MB. Silk fibroin-based nanoparticles for drug delivery. Int J Mol Sci 2015;16:4880-903.

Lewis GA, Mathieu D, Phan-Tan-Luu R. Pharmaceutical experimental design. New York: Marcel Dekker; 1999.

Min BM, Lee G, Kim SH, Nam YS, Lee TS, Park WH. Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. Biomaterials 2004;25:1289-97.

Kundu J, Chung YI, Kim YH, Tae G, Kundu SC. Silk fibroin nanoparticles for cellular uptake and control release. Int J Pharm 2010;388:242-50.

Shi P, Goh JC. Release and cellular acceptance of multiple drugs loaded silk fibroin particles. Int J Pharm 2011;420:282-9.

Wu P, Liu Q, Li R, Wang J, Zhen X, Yue G, et al. Facile preparation of paclitaxel-loaded silk fibroin nanoparticles for enhanced antitumor efficacy by locoregional drug delivery. ACS Appl Mater Interfaces 2013;5:12638-45.

Zhang YQ, Shen WD, Xiang RL, Zhuge LJ, Gao WJ, Wang WB. Formation of silk fibroin nanoparticles in a water-miscible organic solvent and their characterization. J Nanopart Res 2007;9:885-900.

Pham DT, Saelim N, Tiyaboonchai W. Crosslinked fibroin nanoparticles using EDC or PEI for drug delivery: physicochemical properties, crystallinity and structure. J Mater Sci 2018. Doi:10.1007/s10853-018-2635-3

Sakhare SS, Yadav AV, Jadhav PD. Design, development and characterization of mucoadhesive gastro spheres of carvedilol. Int J Appl Pharm 2016;8:37-42.

Kumar S, Singh SK. In silico-in vitro-in vivo studies of experimentally designed carvedilol loaded silk fibroin-casein nanoparticles using physiological based pharmacokinetic model. Int J Biol Macromol 2017;96:403-20.

Rao KV, Venkatchalam VV. Sustained release mucoadhesive microcapsules of ramipril by ionic gelation technique: formulation design, optimization and characterization. Int J Pharm Pharm Sci 2017;9:128-41.

Gohel M, Purohit A, Patel A, Hingorani L. Optimization of bacoside a loaded SNEDDS using D-optimal mixture design for enhancement insolubility and bioavailability. Int J Pharm Pharm Sci 2016;8:213-20.

Thang LQ, Hanh ND, Duong DQ. Study on cause-effect relations and optimization of exemestane-loaded nanostructured lipid carriers. Int J Pharm Pharm Sci 2017;9:68-74.

Singh S, Singla YP, Arora S. Statistical, diagnostic and response surface analysis of nefopam hydrochloride nanospheres using 35 Box-Behnken design. Int J Pharm Pharm Sci 2015;7:89-101.

Montgomery DC. Design and analysis of experiments. 5th ed. New York: Wiley; 2001.




About this article

Title

DESIGN OF EXPERIMENTS MODEL FOR THE OPTIMIZATION OF SILK FIBROIN BASED NANOPARTICLES

Keywords

design of experiments; DOE; D-optimal; optimization; validation; fibroin; nanoparticles

DOI

10.22159/ijap.2018v10i5.28139

Date

08-09-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 10, Issue 5 (Sep-Oct), 2018 Page: 195-201

Online ISSN

0975-7058

Authors & Affiliations

Duy Toan Pham
Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand
Thailand

Nuttawut Saelim
Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand
Thailand

Waree Tiyaboonchai
Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, Thailand The Center of Excellence for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education, Ministry of Education, Bangkok, Thailand The Center of Excellence in Medical Biotechnology, Naresuan University, Phitsanulok 65000, Thailand
Thailand


Refbacks

  • There are currently no refbacks.