• P. Annamalai Centre for Advanced Studies in Botany, University of Madras, Chennai - 600025, India.
  • P. Balashanmugam Centre for Advanced Studies in Botany, University of Madras, Chennai - 600025, India.
  • P. T. Kalaichelvan Centre for Advanced Studies in Botany, University of Madras, Chennai - 600025, India.


Objective: The present study was aimed to screen silver nanoparticles (AgNPs) using different plant extracts and also to study their antimicrobial property against different human pathogens.

Methods: Nine different plants, namely Parthenium hispidum, Vinga rose, Catheranthus roseus, Phyllanthus amarus, Azadirachta indica, Jatropa curcas, Tectona grandis, Ocimum sanctum, and Peltophorum pterocarpum were screened for the synthesis of AgNPs. The AgNPs were synthesized using leaf extracts and was well characterized using a UV-Visible spectrophotometer, transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD) and zeta potential measurement. The AgNPs was tested for their antibacterial and antifungal efficacy using agar well diffusion method.

Results: Among the nine different plant extracts screened, AgNPs synthesized using Peltophorum pterocarpum leaf extract showed good stability even after one month with maximum absorption spectra of 425 nm. The synthesized AgNPs was found to be spherical in shape with an average size ranging from 20 to 60 nm. The EDX spectrum reveals the presence of silver peaks and the XRD spectrum confirms the crystalline nature of AgNPs. A Maximum zone of inhibition of 18.04±0.74 was found when the synthesized AgNPs was tested against B. subtilis, and 12.34±0.31 against A. niger when the concentration was AgNPs was maintained at 100 µg/ml.

Conclusion: The results of the present study conclude that the AgNPs synthesized using Peltophorum pterocarpum leaf extracts is found to be stable and possesses broad-spectrum antibacterial activity against different tested pathogens.

Keywords: Antibacterial, Antifungal, Plant extracts, Silver nanoparticles


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