PHYTOSYNTHESIS OF SILVER NANOPARTICLES USING HYGROPHILA AURICULATA LEAF EXTRACT AND ASSESSMENT OF THEIR ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES


Bharathi S., S. Kumaran, G. Suresh, B. Ramesh, M.s. Nalina Sundari

Abstract


Objective: In this study, the phytosynthesis of silver nanoparticles (AgNPs) using leaf extracts of Hygrophila auriculata (HA) and their biological activities was investigated.

Methods: The synthesis of AgNPs was done by using the green synthetic method and analyzed by UV-Visible spectroscopy, X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and further characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). The antibacterial activity of HA AgNPs was studied by agar well diffusion method and micro broth dilution method for determination of minimum inhibitory concentration (MIC). The antioxidant potentials of HA AgNPs were detected by Diphenyl-1-picryl hydrazyl radical scavenging assay (DPPH), Phosphomolybdenum assay, reducing power assay.

Results: The prepared HA AgNPs showed characteristic absorption peak at 420 nm in the UV-Vis spectrum. FTIR spectra had shown that the biomolecules present in leaf extract were responsible for the reduction and capping material of silver nanoparticles. XRD study showed the particles found to be crystalline in nature, with a face-centered cubic (fcc) structure. TEM and DLS results revealed that the AgNPs were mostly spherical with an average size ranging from ~15-115 nm with a mean diameter of 40.96 nm. The HA AgNPs showed good antibacterial activity and MIC against Staphylococcus aureus (ATCC 6538), Bacillus cereus (NCIM 2106), Pseudomonas aeruginosa (ATCC 9027) and Escherichia coli (ATCC 8739). In agar well diffusion method, the maximum zone of inhibition was found against Pseudomonas aeruginosa (ATCC 9027) with 18 mm and minimum zone of inhibition was found to be against Escherichia coli (ATCC 8739) with 13 mm. The MIC of the HA AgNp was found to be 5µg against all the test organisms. In addition, the Diphenyl-1-picryl hydrazyl radical scavenging assay (DPPH), Phosphomolybdenum assay, reducing power assay revealed they can be used as the potential scavenger against deleterious damages caused by the free radicals.

Conclusion: The present study explored that Hygrophila auriculata which are efficient producers of AgNPs and could act as safe and cost-effective with potential antibacterial and antioxidant activities. These findings encourage studying HA AgNP further for their potential biological applications.


Keywords


Phytosynthesis, Hygrophila auriculata, Silver nanoparticles, Antibacterial, Antioxidant

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About this article

Title

PHYTOSYNTHESIS OF SILVER NANOPARTICLES USING HYGROPHILA AURICULATA LEAF EXTRACT AND ASSESSMENT OF THEIR ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES

Keywords

Phytosynthesis, Hygrophila auriculata, Silver nanoparticles, Antibacterial, Antioxidant

DOI

10.22159/ijap.2018v10i6.28605

Date

04-09-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Articles In Press [Scheduled in Nov-Dec 2018] Page: 112-118

Online ISSN

0975-7058

Authors & Affiliations

Bharathi S.
Department of Zoology, Queen Mary’s College, Chennai-600004, Tamil Nadu
India

S. Kumaran
Department of Microbiology, Sri Sankara Arts and Science College, Kanchipuram - 631561,Tamil Nadu
India

G. Suresh
Department of Microbiology, Sri Sankara Arts and Science College, Kanchipuram-631561, Tamil Nadu
India

B. Ramesh
Department of Biotechnology, Sri Sankara Arts and Science College, Kanchipuram-631561, Tamil Nadu
India

M.s. Nalina Sundari
Department of Zoology, Queen Mary’s College, Chennai-600004, Tamil Nadu
India


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