ENRICHMENT OF IN VIVO EFFICACY OF CATECHIN RICH EXTRACT WITH THE APPLICATION OF NANOTECHNOLOGY


Monika P., Basavaraj B. V., Chidambara Murthy K. N., Ahalya N., Bharath S.

Abstract


Objective: The primary goal of this study was to convert a natural catechin-rich extract into nanoparticles by using a biodegradable and non-toxic polymer Eudragit L 100 to address the various biopharmaceutical problems of catechin.

Methods: Nanoparticles were prepared by emulsion solvent evaporation technique using Eudragit L 100 in increasing concentration. Optimization of processing conditions like a selection of organic solvents, diluent and surfactant concentrations, drug and polymer ratio and method of drying to increase the biological efficiency were duly attempted. Parameters such as dynamic light scattering, zeta potential, SEM and energy-dispersive X-ray spectroscopy were assessed for the evaluation of nanoparticles.

Results: The entrapment efficiency was found to be between 35-45 % with methanol compared to other organic solvents. The zeta potential values of all the formulations were in the range of±30 mV to±60 mV) which confirms moderate to good stability. A rapid or ‘burst’ effect of the drug release in pH 6.8 buffer showing 92 % in the first 30 min which gradually decreased to 52 % by the end of 180 min but in the pH 7.4, the release was found to be moderate. SEM and DLS indicated particles were of spherical shape lying in a nanometer range of 100 to 200 nm with a proportional influence of polymer on the particles size.

Conclusion: Nanoformulations were found to be more stable and confirmed the presence of major elements such as carbon and oxygen. The findings collectively indicate that it may be worthwhile to apply nanotechnology for the design of an advanced oral dosage form for an enhanced bioavailability and biological efficacy.

Keywords


Catechin rich extract, Bioavailability, Bioefficacy, Pharmacokinetics, Eudragit L 100, Nanoformulation

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About this article

Title

ENRICHMENT OF IN VIVO EFFICACY OF CATECHIN RICH EXTRACT WITH THE APPLICATION OF NANOTECHNOLOGY

Keywords

Catechin rich extract, Bioavailability, Bioefficacy, Pharmacokinetics, Eudragit L 100, Nanoformulation

DOI

10.22159/ijap.2018v10i5.29569

Date

08-09-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 10, Issue 5 (Sep-Oct), 2018 Page: 281-288

Online ISSN

0975-7058

Authors & Affiliations

Monika P.
Department of Biotechnology, M. S. Ramaiah Institute of Technology, MSRIT Post, Bengaluru 560054, India

Basavaraj B. V.
Department of Pharmaceutics, Faculty of Pharmacy, M. S. Ramaiah University of Applied Sciences, MSRIT, Post, Bengaluru 560054, India

Chidambara Murthy K. N.
Division of Research and Patents, Central Research Laboratory, M. S. Ramaiah Medical College and Teaching Hospital, MSRIT Post, Bengaluru 560054, India

Ahalya N.
Department of Biotechnology, M. S. Ramaiah Institute of Technology, MSRIT Post, Bengaluru 560054, India

Bharath S.
Department of Pharmaceutics, Faculty of Pharmacy, M. S. Ramaiah University of Applied Sciences, MSRIT, Post, Bengaluru 560054, India


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