DEVELOPMENT OF HERBAL CAPSULES CONTAINING MULBERRY LEAF AND BLACK TEA EXTRACTS USING THE MODIFIED LIQUISOLID TECHNIQUES
Objective: The objective of this study was to develop the capsules containing mulberry leaf extract (MLE) and black tea extract (BTE).
Methods: MLE and BTE were prepared by maceration and determined for phytochemicals, in vitro alpha-amylase and alpha-glucosidase inhibitory activities using the enzymatic colorimetric assay. The granules of MLE and BTE were prepared by the application of liquisolid technique and evaluated for the flow properties. The selected granule formulation was filled into the hard gelatin capsule and evaluated for weight variation and disintegration.
Results: The yields of MLE and BTE solid extracts were 8.12 and 4.23% w/w, respectively. Total phenolic and total flavonoid contents were 32.46±5.22 mg TAE/g DW and 44.03±3.37 mg QE/g DW for MLE and 244.66±23.28 mg TAE/g DW and 214.43±3.22 mg QE/g DW for BTE, respectively. The IC50 for alpha-amylase of MLE and BTE were 0.69±0.04 and 3.34±0.08 mg/ml, respectively; whereas those for alpha-glucosidase of MLE and BTE were 0.67±0.42 and 0.43±0.15 mg/ml, respectively. The granule prepared with MCC and silica at the ratio of 20:1 showed the highest flowability. The weight variation of the prepared MLE and BTE capsules was within the range of the limitation criteria of ±5%. The average disintegration time of capsules was 1.1±0.1 min.
Conclusion: Herbal capsules of MLE and BTE were successfully prepared. The suitable carrier and coating were MCC and silica with a ratio of 20:1. This study revealed the potential application of liquisolid technique as a tool to produce a capsule of herbal crude extracts.
2. Xu Y, Zhang M, Wu T, Dai SD, Xu J, Zhou Z. The anti-obesity effect of green tea polysaccharides, polyphenols and caffeine in rats fed with a high-fat diet. Food Funct 2015;6:297.
3. Bollapragada MK, Shantaram M, R SK. Obesity: development, epidemiology, factors affecting, quantity, health hazards, management and natural treatment-a review. Int J Pharm Pharm Sci 2017;9:12-26.
4. Jeong JH, Lee NK, Cho SH, Jeong DY, Jeong YS. Enhancement of 1-deoxynojirimycin content and ?-glucosidase inhibitory activity in the mulberry leaf using various fermenting microorganisms isolated from Korean traditional fermented food. Biotechnol Bioprocess Eng 2014;19:1114-8.
5. Thaipitakwong T, Numhom S, Aramwit P. Mulberry leaves and their potential effects against cardiometabolic risks: a review of chemical compositions, biological properties and clinical efficacy. Pharm Biol 2018;56:109-18.
6. MacKenzie T, Leary L, Brooks WB. The effect of an extract of green and black tea on glucose control in adults with type 2 diabetes mellitus: a double-blind randomized study. Metab Clin Exp 2007;56:1340–4.
7. Javadzadeha Y, Musaalrezaei L, Nokhodchi A. Liquisolid technique as a new approach to sustain propranolol hydrochloride release from tablet matrices. Int J Pharm 2008;362:102-8.
8. Lu M, Xing H, Jiang J, Chen X, Yang T, Wang D, et al. Liquisolid technique and its applications in pharmaceutics. Asian J Pharm Sci 2017;12:115–23.
9. Banlangsawan N, Sripanidkulchai B, Sanoamuang N. Investigation of antioxidative, antityrosinase and cytotoxic effects of an extract of irradiated oyster mushroom. Songklanakarin J Sci Technol 2016;38:31–9.
10. Singleton VL, Orthofer R, Lamuela Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin–ciocalteau reagent. Methods Enzymol 1999;299:152–78.
11. Woisky R, Salatino A. Analysis of propolis: some parameters and procedures for chemical quality control. J Apic Res 1998;37:99–105.
12. Brand Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 1995;28:25–30.
13. Elya B, Handayani R, Sauriasari R, Azizahwati, Hasyyati US, Permana IT, et al. Antidiabetic activity and phytochemical screening of extracts from Indonesian plants by inhibition of alpha-amylase, alpha-glucosidase and dipeptidyl peptidase IV. Pak J Biol Sci 2015;18:279-84.
14. Si MM, Lou JS, Zhou CX, Shen JN, Wu HH, Yang B, et al. Insulin releasing and alpha-glucosidase inhibitory activity of ethyl acetate fraction of Acorus calamus in vitro and in vivo. J Ethnopharmacol 2010;128:154-9.
15. United States Pharmacopeia and National Formulary (USP 39-NF 34). Rockville, MD. United States Pharmacopeial Convention; 2015.
16. Carr RL. Evaluating flow properties of solids. Chem Eng 1965;72:163-8.
17. Surini S, Diandra DM. Formulation of the mulberry leaf (Morus alba L.) extracts hydrogel beads using cross-linked pectin. Int J Appl Pharm 2017;9:159-62.
18. Roy N, Bhattacharjee K, Bandhopadhyaya S, Chatterjee S, Saha AK, Chatterjee A, et al. Role of black tea in type 2 diabetes mellitus and metabolic syndrome. Am J Phytomed Clin Ther 2015;3:570-4.
19. Gramza A, Pawlak Lemañska K, Korczak J, W?sowicz E, Rudzinska M. Tea extracts as free radical scavengers. Pol J Environ Stud 2005;14:861-7.
20. Adisakwattana S, Ruengsamran T, Kampa P, Sompong W. In vitro inhibitory effects of plant-based foods and their combinations on intestinal ?-glucosidase and pancreatic ?-amylase. BMC Complementary Altern Med 2012;12:110.
21. Flaczyk E, Kobus Cisowska J, Przeor M, Korczak J, Remiszewski M, Korbas E, et al. Chemical characterization and antioxidative properties of Polish variety of Morus alba L. leaf aqueous extracts from the laboratory and pilot-scale processes. Agric Sci 2013;4:141-7.
22. Radojkovic MM, Zekovic ZP, Vidovic SS, Kocar DD, Maskovic PZ. Free radical scavenging activity and total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extract. Hem Ind 2012;66:547–52.
23. Zou Y, Liao S, Shen W, Liu F, Tang C, Chen CYO, et al. Phenolics and antioxidant activity of mulberry leaves depend on cultivar and harvest month in southern China. Int J Mol Sci 2012;13:16544-53.
24. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64:555–9.
25. Abdeltaif SA, SirElkhatim KA, Hassan AB. Estimation of phenolic and flavonoid compounds and antioxidant activity of spent coffee and black tea (processing) waste for potential recovery and reuse in sudan. Recycling 2018;3:27.
26. Pereira VP, Knor FJ, Vellosa JCR, Beltrame FL. Determination of phenolic compounds and antioxidant activity of green, black and white teas of Camellia sinensis (L.) Kuntze, Theaceae. Rev Bras Pl Med Campinas 2014;16:490-8.
27. Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 2005;53:4290–302.
28. Chon SU, Kim YM, Park YJ, Heo BG, Park YS, Gorinstein S. Antioxidant and antiproliferative effects of methanol extracts from raw and fermented parts of mulberry plant (Morus alba L.). Eur Food Res Technol 2009;230:231-7.
29. Haslam E. Thoughts on thearubigins. Phytochemistry 2003;64:61–73.
30. Leung LK, Su Y, Chen R, Zhang Z, Huang Y, Chen ZY. Theaflavins in black tea and catechins in green tea are equally effective antioxidants. J Nutr 2001;131:2248-51.
31. Pan H, Gao Y, Tu Y. Mechanisms of body weight reduction by black tea polyphenols. Molecules 2016;21:1659.
32. Chang YC, Yang MY, Chen SC, Wang CJ. Mulberry leaf polyphenol extract improves obesity by inducing adipocyte apoptosis and inhibiting preadipocyte differentiation and hepatic lipogenesis. J Funct Foods 2016;21:249–62.
33. Satoh T, Igarashi M, Yamada S, Takahashi N, Watanabe K. Inhibitory effect of black tea and its combination with acarbose on small intestinal ?-glucosidase activity. J Ethnopharmacol 2015;161:147–55.
34. Habeeb MN, Naik PR, Moqbel FS. Inhibition of ?-glucosidase and ?-amylase by Morus alba Linn leaf extracts. J Pharm Res 2012;5:285-9.
35. Nickavar B, Mosazadeh G. Influence of three Morus species extracts on ?-amylase activity. Iran J Pharm Res 2009;8:115-9.
36. Striegel L, Kang B, Pilkenton SJ, Rychlik M, Apostolidis E. Effect of black tea and black tea pomace polyphenols on ?-glucosidase and ?-amylase inhibition, relevant to type 2 diabetes prevention. Front Nutr 2015;2:3.
37. Yang Z, Wang Y, Wang Y, Zhang Y. Bioassay-guided screening and isolation of ?-glucosidase and tyrosinase inhibitors from leaves of Morus alba. Food Chem 2012;131:617–25.
38. Hao W, Wang M, Lv M. The inhibitory effects of Yixing black tea extracts on ?–glucosidase. J Food Biochem 2017;41:e12269.
39. Oh J, Jo SH, Kim JS, Ha KS, Lee JY, Choi HY, et al. Selected tea and tea pomace extracts inhibit intestinal ?-glucosidase activity in vitro and postprandial hyperglycemia in vivo. Int J Mol Sci 2015;16:8811-25.
40. S JP, Sabina EP. Global current trends in natural products for diabetes management: a review. Int J Pharm Pharm Sci 2016;8:20-8.
41. Takahiko A, Kouichi T, Hiromi O, Hironori T. Maltase, sucrase and ?–amylase inhibitory activity of Morus leaves extract. Food Preservation Sci 2004;30:2239.
42. Hong HC, Li SL, Zhang XQ, Ye WC, Zhang QW. Flavonoids with ?-glucosidase inhibitory activities and their contents in the leaves of Morus atropurpurea. Chin Med 2013;8:19.
43. Oku T, Yamada M, Nakamura M, Sadamori N, Nakamura S. Inhibitory effects of extractives from leaves of Morus alba on human and rat small intestinal disaccharidase activity. Br J Nutr 2006;95:933–8.
44. Hara Y, Honda M. The inhibition of ?-amylase by tea polyphenols. Agric Biol Chem 1990;54:1939-45.
45. Koh LW, Wong LL, Loo YY, Kasapis S, Huang D. Evaluation of different teas against starch digestibility by mammalian glycosidases. J Agric Food Chem 2010;58:148–54.
46. Matsui T, Tanaka T, Tamura S, Toshima A, Tamaya K, Miyata Y, et al. ?-Glucosidase inhibitory profile of catechins and theaflavins. J Agric Food Chem 2007;55:99–105.
47. Jeon SY, Oh S, Kim E, Imm JY. ?-Glucosidase inhibition and antiglycation activity of laccase catalyzed catechin polymers. J Agric Food Chem 2013;61:4577–84.
48. Bischoff H, Puls W, Krause HP, Schutt H, Thomas G. Pharmacological properties of the novel glucosidase inhibitors BAY m 1099 (miglitol) and BAY o 1248. Diabetes Res Clin Pract 1985;1:53–62.
49. Rosenstock J, Brown A, Fischer J, Jain A, Littlejohn T, Nadeau D, et al. Efficacy and safety of acarbose in metformin-treated patients with type2 diabetes. Diabetes Care 1998;21:2050–5.
50. Kwon YI, Apostolidis E, Shetty K. Inhibitory potential of wine and tea against ?-amylase and alpha-glucosidase for management of hyperglycemia linked to type 2 diabetes. J Food Biochem 2008;32:15–31.
51. Tan SB, Newton JM. Powder flowability as an indication of capsule filling performance. Int J Pharm 1990;61:145-55.
52. Lu M, Xing H, Yang T, Yu J, Yang Z, Sun Y, et al. Dissolution enhancement of tadalafil by liquisolid technique. Pharm Dev Technol 2017;22:77–89.
53. Vranikova B, Gajdziok J, Vetchy D. Determination of flowable liquid retention potential of aluminometasilicate carrier for liquisolid systems preparation. Pharm Dev Technol 2015;20:839–44.
54. Spireas S, Wang T, Grover R. Effect of powder substrate on the dissolution properties of methylchlorothiazide liquisolid compacts. Drug Dev Ind Pharm 1999;25:163–8.
55. Javadzadeh Y, Siahi MR, Asnaashari S, Nokhodchi A. An investigation of physicochemical properties of piroxicam liquisolid compacts. Pharm Dev Technol 2007;12:337–43.
56. Naveen C, Shastri N, Tadikonda RR. Use of the liquisolid compact technique for improvement of the dissolution rate of valsartan. Acta Pharm Sin B 2012;2:502–8.
57. Hentzschel CM, Sakmann A, Leopold CS. Suitability of various excipients as carrier and coating materials for liquisolid compacts. Drug Dev Ind Pharm 2011;37:1200–7.
58. Rowe RC, Sheskey PJ, Quinn ME. Handbook of pharmaceutical excipients. 6th ed. Gurnee (IL): Pharmaceutical Press; 2009.
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