ANTIBACTERIAL EFFICACY OF GRAPE SEED EXTRACT CONTAINING 2.9% TANNIN AGAINST ENTEROCOCCUS FAECALIS BIOFILM
Keywords:Grape seed extract, Biofilm, Enterococcus faecalis
Objective: The aim of this study was to observe the antibacterial efficacy of grape seed extract (GSE) against Enterococcus faecalis biofilm.
Methods: A biofilm of E. faecalis American Type Culture Collection (ATCC) 29212 strain was prepared using sterile cellulose nitrate filter membrane
incubated on brain heart infusion agar at 37°C for 72 h under aerobic condition. Each membrane containing E. faecalis biofilm was added to three
tubes of phosphate-buffered saline (control), three tubes of GSE, and three tubes of 2% clorhexidine. The number of viable DNA cells was measured
using real-time polymerase chain reaction. The data were statistically analyzed using non-parametric Kruskal–Wallis test and Mann–Whitney U-test.
Results: GSE had antibacterial efficacy against E. faecalis biofilm. The difference in the amount of E. faecalis DNA between all groups was statistically
Conclusion: GSE has antibacterial efficacy against E. faecalis biofilm.
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