ANTIBACTERIAL EFFICACY OF GRAPE SEED EXTRACT CONTAINING 2.9% TANNIN AGAINST ENTEROCOCCUS FAECALIS BIOFILM

  • SHELVY SOETANTO Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia.
  • ENDANG SUPRASTIWI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia
  • DINI ASRIANTI Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia

Abstract

Objective: The aim of this study was to observe the antibacterial efficacy of grape seed extract (GSE) against Enterococcus faecalis biofilm.
Methods: A biofilm of E. faecalis American Type Culture Collection (ATCC) 29212 strain was prepared using sterile cellulose nitrate filter membrane
incubated on brain heart infusion agar at 37°C for 72 h under aerobic condition. Each membrane containing E. faecalis biofilm was added to three
tubes of phosphate-buffered saline (control), three tubes of GSE, and three tubes of 2% clorhexidine. The number of viable DNA cells was measured
using real-time polymerase chain reaction. The data were statistically analyzed using non-parametric Kruskal–Wallis test and Mann–Whitney U-test.
Results: GSE had antibacterial efficacy against E. faecalis biofilm. The difference in the amount of E. faecalis DNA between all groups was statistically
significant (p=0.05).
Conclusion: GSE has antibacterial efficacy against E. faecalis biofilm.

Keywords: Grape seed extract, Biofilm, Enterococcus faecalis

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SOETANTO, S., SUPRASTIWI, E., & ASRIANTI, D. (2019). ANTIBACTERIAL EFFICACY OF GRAPE SEED EXTRACT CONTAINING 2.9% TANNIN AGAINST ENTEROCOCCUS FAECALIS BIOFILM. International Journal of Applied Pharmaceutics, 11(1), 54-58. https://doi.org/10.22159/ijap.2019.v11s1.165
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