THE EFFECT OF LUNASIN ON LIVER HISTOPATHOLOGY OF MICE INDUCED BY AZOXIMETHANE (AOM) AND DEXTRAN SODIUM SULPHATE (DSS)
Keywords:Azoxymethane, Dextran sodium sulfate, Liver, Mice, Lunasin
Objective: Azoximethane (AOM) and dextran sodium sulfate (DSS) are commonly used to induce colorectal cancer, but have side effects on the liver. AOM can induce hepatic histopathological changes such as the appearance of microvesicular steatosis and necrosis. DSS plays a role in causing inflammation and indirect scarring through colitis. This study aimed to determine in mice the effect of lunasin on the liver histopathology induced by AOM and DSS.
Methods: This was an experimental study conducted in BALB/c mice. The data analyzed were the results of quantification of foci of necrosis, steatosis, and dysplasia from liver preparations of mice given lunasin extract at doses of 20, 30, and 40 mg/kg and control group.
Results: This study showed a significant decrease in the number of foci of necrosis in the group given 30 mg/kg lunasin, with a mean score of 9.0±3.4 compared with the control group mean score of 14.0±0.8 (P = 0.017). There was also a significant decrease in the number of foci of steatosis in the group given 30 mg/kg lunasin with a mean score of 3.8±1.3 compared with the control group mean score of 11.5±1.9 (P = 0.002).
Conclusion: The results of this study indicate that lunasin at doses above 30 mg/kg can inhibit the formation of foci of necrosis and steatosis induced in mouse liver by AOM and DSS.
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