THE COMBINATION OF EXERCISE AND ASCORBIC ACID DECREASE BLOOD GLUCOSE LEVEL AND TEND TO AMELIORATE PANCREATIC ISLETS AREA ON HIGH CARBOHYDRATE DIET RATS
Objective: This study is conducted to determine the protective effects of physical exercise and ascorbic acid on increasing blood glucose (BG) levels and islet pancreatic area in high-carbohydrate (HC) diet rats.
Methods: A total of 20 rats were divided into four groups: Control group which was a HC and treatment groups which were HC plus exercise (HCEx), HC plus ascorbic acid (HCAs), and HCEx and ascorbic acid (HCExAs). The duration of treatment was 9 weeks. Swimming to exercise held 6 times a week and ascorbic acid dose was 9 mg.
Results: It showed that the smallest body weight was HCEx group. BG difference (before and after treatment = BG diff) had a significant difference (p=0.021) among groups, and the lowest level of BG diff was HCEx group. HCAs had the biggest BG diff. However, there was no significantly difference among groups on islet pancreatic area, but HC group had the largest area.
Conclusion: This study suggests that a combination of exercise and ascorbic acid on HC diet subject may regulate BG level compared to the exercise or ascorbic acid alone. However, they do not influence pancreatic islet area.
2. Guariguata L, Whiting DR, Hambleton I, Beagley J, Linnenkamp U, Shaw JE, et al. Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes Res Clin Pract 2014;103:137-49.
3. Benkar AP, Kanase SB. Effect of aerobic exercises and resistance training on glucose levels in Type 2 diabetic subjects. Asian J Pharm Clin Res 2017;10:360.
4. And?l M, N?mcová V, Pavlíková N, Urbanová J, Cecháková M, Havlová A, et al. Factors causing damage and destruction of beta-cells of the islets of Langerhans in the pancreas. Vnitr Lek 2014;60:684-90.
5. Zhao LG, Shu XO, Li HL, Zhang W, Gao J, Sun JW, et al. Dietary antioxidant vitamins intake and mortality: A report from two cohort studies of chinese adults in Shanghai. J Epidemiol 2017;27:89-97.
6. Herawati L, Anshori N, Harjanto H. Peningkatan performa atlet selam dengan pemberian antioksidan. J Iptek Olahraga 2012;14:258-267.
7. Adeyi AO, Idowu BA, Mafiana CF, Oluwalana SA, Ajayi OL, Akinloye OA, et al. Rat model of food-induced non-obese-Type 2 diabetes mellitus: Comparative pathophysiology and histopathology. Int J Physiol Pathophysiol Pharmacol 2012;4:51-8.
8. Kusumawati D. Bersahabat Dengan Hewan Coba. 1st ed. Yogyakarta: UGM Press-Badan Penerbit dan Publikasi Universitas Gadjah Mada; 2004.
9. Kanter M. High-quality carbohydrates and physical performance: Expert panel report. Nutr Today 2018;53:35-9.
10. Ferreira GA, Felippe LC, Silva RL, Bertuzzi R, De Oliveira FR, Pires FO, et al. Effect of pre-exercise carbohydrate availability on fat oxidation and energy expenditure after a high-intensity exercise. Braz J Med Biol Res 2018;51:e6964.
11. Colberg SR, Sigal RJ, Yardley JE, Riddell MC, Dunstan DW, Dempsey PC, et al. Physical activity/exercise and diabetes: A position statement of the American diabetes association. Diabetes Care 2016;39:2065-79.
12. Lee D, Hwang W, Artan M, Jeong DE, Lee SJ. Effects of nutritional components on aging. Aging Cell 2015;14:8-16.
13. Herawati L, Irwadi I, Sari GM, Harjanto. Peningkatan Protein Pengangkut Gula (Glut4) Dengan Diet Tinggi Karbohidrat dan Latihan Fisik Teratur. Proceeding Kongres Nas. IAIFI XVI, Simp. Seminar Nasional, dan Work. XXIV Interak. Padang: Holistik antara Org. dan
Lingkung. untuk Kualitas Hidup yang Lebih Baik Ketahanan Pangan, Kesehatan, dan Prestasi Olahraga; 2015. p. 796-806.
14. Vasudevan S, Hirsch IB. Interference of intravenous Vitamin C with blood glucose testing. Diabetes Care 2014;37:e93-4.
15. Sharma E, Resta C, Park P. A case of factitious hyperglycemia in a patient on intravenous ascorbic acid. Case Rep Endocrinol 2018;2018:7063137.
16. Dakhale GN, Chaudhari HV, Shrivastava M. Supplementation of Vitamin C reduces blood glucose and improves glycosylated hemoglobin in Type 2 diabetes mellitus: A randomized, double-blind study. Adv Pharmacol Sci 2011;2011:195271.
17. Leloup C, Tourrel-Cuzin C, Magnan C, Karaca M, Castel J, Carneiro L, et al. Mitochondrial reactive oxygen species are obligatory signals for glucose-induced insulin secretion. Diabetes 2009;58:673-81.
18. McConell GK, Kingwell BA. Does nitric oxide regulate skeletal muscle glucose uptake during exercise? Exerc Sport Sci Rev 2006;34:36-41.
19. Katz A. Modulation of glucose transport in skeletal muscle by reactive oxygen species. J Appl Physiol (1985) 2007;102:1671-6.
20. Almeida FN, Proença AR, Chimin P, Marçal AC, Bessa-Lima F, Carvalho CR, et al. Physical exercise and pancreatic islets: Acute and chronic actions on insulin secretion. Islets 2012;4:296-301.
21. Sheng Q, Xiao X, Prasadan K, Chen C, Ming Y, Fusco J, et al. Autophagy protects pancreatic beta cell mass and function in the setting of a high-fat and high-glucose diet. Sci Rep 2017;7:16348.
22. Campos C. Chronic hyperglycemia and glucose toxicity: Pathology and clinical sequelae. Postgrad Med 2012;124:90-7.
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