OPTIMIZATION OF PCL-PEG-PCL TRIBLOCK COPOLYMER MICELLES AS HYDROPHOBIC DRUG CARRIER WITH A 22 FULL FACTORIAL DESIGN
This study aims to optimize PCL-PEG-PCL (PCEC) triblock copolymer micelles as hydrophobic drug carrier, simvastatin (SV). PCEC triblock copolymer was prepared by ring opening polymeritation method (ROP) with different ɛCL/PEG ratio (2-5). SV was incorporated into the PCEC triblock copolymer micelles with concentration of 2.5-10% b/b by the solvent evaporation method (film formation). The factors ratio of ɛCL/PEG and concentration of SV effect on the responses particle size (PS), polidispersity index (PI) and entrapment efficiency (EE) was assessed using 22 full factorial design method. The test results were analyzed using Design-Expert software to obtain the optimum formula. The optimization results showed that the optimum formula chosen were ɛCL/PEG ratio of 5 and SV concentration of 10% w/w, with the PS, PI dan EE value were 322,1±3.51 nm, 0,471±0,09 and 87,08±1,17%, respectively. The 22 full factorial design has been proven to be used as an optimization method to determine the optimum formula of SV-loaded PCEC triblock copolymer micelles with good result of the PS, PI and EE responses.
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