• S. GOKUL BRINDHA Research Scholars of Department of Botany, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India
  • V. S. HANSIYA Research Scholars of Department of Botany, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India
  • P. UMA MAHESWARI Research Scholars of Department of Botany, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India
  • N. GEETHA Faculty of Department of Botany, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India


Objective: The objective of the work was to evaluate the efficacy of anti-biofilm activity of green synthesized silver and iron oxide nanoparticles comparatively.

Methods: Nanoparticles were synthesized using a rapid, single-step and completely by a green biosynthetic method employing aqueous leaf extracts of Moringa oleifera Lam. The synthesized nanoparticles were characterized by UV-Vis spectrophotometry and X-Ray Diffraction. Bacterial strains used in this study included Staphylococcus epidermidis and Pseudomonas aeruginosa. The biofilm reduction was evaluated through ring test using crystal violet as a staining agent.

Results: Colour change was observed after half an hour, which indicated the formation of silver and iron nanoparticles. Synthesis of nanoparticles was confirmed by UV and XRD. The anti-biofilm forming ability of AgNPs and FeNPs were compared with standard antibiotic. It was found that FeNPs showed more biofilm destruction ability (58%) for S. compared to P. aeruginosa (50%) and standard (30%). Whereas, AgNPs displayed significant biofilm destruction ability (78%) for P. aeruginosa compared to S. epidermidis (43%) and standard (34%).

Conclusion: Based on the results obtained in this investigation, it is concluded that FeNPs have anti-biofilm activity against S. epidermidis whereas AgNPs have anti-biofilm activity against P. aeruginosa.

Keywords: Anti-biofilm, Silver nanoparticles, Iron nanoparticles, P. aeruginosa, S. epidermidis


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How to Cite
BRINDHA, S. G., HANSIYA, V. S., MAHESWARI, P. U., & GEETHA, N. (2019). A COMPARATIVE IN VITRO ANTI-BIOFILM EFFICACY OF PHYTOSYNTHESIZED IRON AND SILVER NANOPARTICLES. International Journal of Applied Pharmaceutics, 12(1), 72-76. https://doi.org/10.22159/ijap.2020v12i1.35047
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