DETERMINATION OF LUPEOL FROM MAKHAEN AT THAILAND AND TAIWAN BY HIGHPERFORMANCE LIQUID CHROMATOGRAPHY

  • SURACHAI TECHAOEI Department of Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12130 Thailand.
  • KHEMJIRA JARMKOM Department of Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12130 Thailand.
  • NAKUNTWALAI WISIDSRI Department of Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12130 Thailand.
  • SURADWADEE THUNGMUNGMEE Department of Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12130 Thailand.
  • WARACHATE KHOBJAI Department of Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, 12130 Thailand

Abstract

Objective: The objective of this research was to investigate the extraction and isolation of chemical compounds of Zanthoxylum limonella Alston
extracts which selected from Chiang Mai and Pa Yao province, Thailand and Taiwan.
Method: There are 3 locations of medicinal plant, Z. limonella Alston was collected, two provinces of northern Thailand, Chiang Mai and Phayao,
including the other country, Taiwan. All of extractions were carried out by methanol. In this experimental study, thin-layer chromatography (TLC) and
high-performance liquid chromatography (HPLC) were measured.
Results: The result was found that the best organic solvent for extraction is methanol. The suitable of extraction was methanol and toluene in the ratio
of 9:1 when developed with TLC and gradient methanol and water when analyzed with HPLC. The potential bioactive compound from crude extracted
was lupeol. Crude extracted from leaf at Chiang Mai was highest lupeol as 5655.72 μg/mg extraction whereas crude extracted from leaf at Phayao
showed this compound as 16, 98.81 μg/mg extraction. In addition, the crude extraction from root and bark of both areas showed the amount of lupeol
less than leaf crude extracted. The further study will focus on antimicrobial activity and biological activity in the near future.
Conclusion: The bioactive compound, lupeol was found in all part of Makhaen in Thailand but did not found in Taiwan seed.

Keywords: Zanthoxylum limonella Alston, Lupeol, Extraction, Medicinal plants

References

1. Pierre MJ, Munyabuhoro M, Emmanuel BS. In vitro antimicrobial
investigation of Zanthoxylum chalybeum stem bark. J Med Plants Res
2013;7:1157-79.
2. Majumder M, Sharma MH, Zaman K, Lyngdoh W. Evaluation of
physic-chemical properties and antibacterial activity of the essentaial
oil obtained from the fruits of Zanthoxylum acanthopodium DC.
Collected from Meghalaya, India. Int J Pharm Sci 2014;6:543-6.
3. Janpen J, Roongtawan S, Warinthorn C. Antioxidant effect of
Zanthoxylum limonella Alston. J Med Plants Res 2012;6:1407-14.
4. Patchanee C, Montinee T, Chamroon L. An allelopathic substrate
isolated from Zanthoxylum limonella Alston fruit. Sci Hortic
2010;125:411-6.
5. Supabphol R, Tangitjareonkun J. Chemical constituents and biological
activities of Zanthoxylum limonella (Rutaceae): A review. Trop J Pharm
Res 2014;13:2119-30.
6. Simanek V. Benzophenanthridine alkaloids. In: Brossi A, editor. The
Alkaloids, Chemistry and Pharmacology. London: Academic Press;
1985. p. 186-240.
7. Tangjitjaroenkun J, Chantarasriwong O, Chavasiri, W. Chemical
constituents of the stems of Zanthoxylum limonella Alston. Phytochem
Lett 2012;5:443-5.
8. Saleem M. Lupeol, a novel anti-inflammatory and anti-cancer dietary
triterpene. Cancer Lett 2009;285:109-15.
9. Wongkattiya N, Akekawatchai C, Sanguansermsri P, Fraser IH,
Pratoomsoot C, Sanguansermsri D. Chemical composition and
biological properties of essential oils from Zanthoxylum rhetsa (Roxb.)
DC and Zanthoxylum limonella Alston. Complement Altern Med
2018;15:12-8.
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How to Cite
TECHAOEI, S., JARMKOM, K., WISIDSRI, N., THUNGMUNGMEE, S., & KHOBJAI, W. (2019). DETERMINATION OF LUPEOL FROM MAKHAEN AT THAILAND AND TAIWAN BY HIGHPERFORMANCE LIQUID CHROMATOGRAPHY. International Journal of Applied Pharmaceutics, 11(5), 49-51. https://doi.org/10.22159/ijap.2019.v11s5.T0045
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