CYTOTOXIC ACTIVITY OF ERIOCAULON CINEREUM R.BR TO MCF-7 AND VERO CELL LINE

  • ARDE TOGA NUGRAHA Department of Pharmacy, Universitas Islam Indonesia, Yogyakarta, Indonesia
  • ASGAR PURNAMA Department of Pharmacy, Universitas Islam Indonesia, Yogyakarta, Indonesia
  • SITI NURUL KOMARIAH Department of Pharmacy, Universitas Islam Indonesia, Yogyakarta, Indonesia
  • HADY ANSHORI T Department of Pharmacy, Universitas Islam Indonesia, Yogyakarta, Indonesia
  • HADY ANSHORI T Department of Pharmacy, Universitas Islam Indonesia, Yogyakarta, Indonesia

Abstract

Objective: The aim of the study was to determine the strength of the Eriocaulon cinereum R.Br plant against breast cancer cells (MCF7) and cytotoxic
against Vero cells.
Methods: E. cinereum R.Br was taken from the province of Bangka Belitung and then extracted in stages with n-hexane, ethyl acetate, and methanol.
Then, the ethyl acetate extract was fractionated using the vacuum liquid chromatography method with dichloromethane and ethyl acetate solvents.
Sample was tested for MCF-7 cells and Vero cells using the MTT method (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). The data
obtained is analyzed by probit SPSS.
Results: The results of this study showed that the best IC50 extract was ethyl acetate extract with an IC50 value of 450.31 μg/ml. Then, ethyl acetate
extract produced dichloromethane fraction with IC50 value of 443.52 μg/ml and ethyl acetate with IC50 value of 214.75 μg/ml. Ethyl acetate fraction
was also tested against Vero cells to see cytotoxic against normal human cells with IC50 679.11 μg/ml

Keywords: Eriocaulon cinereum R.Br, MCF-7, Vero, 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide

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NUGRAHA, A. T., PURNAMA, A., KOMARIAH , S. N., ANSHORI T, H., & HADY ANSHORI T. (2019). CYTOTOXIC ACTIVITY OF ERIOCAULON CINEREUM R.BR TO MCF-7 AND VERO CELL LINE. International Journal of Applied Pharmaceutics, 11(5), 94-96. https://doi.org/10.22159/ijap.2019.v11s5.T0073
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