EVALUATION OF ACETYLCHOLINESTERASE ACTIVITY AND CYTOTOXICITY OF DIFFERENT PARTS OF NELUMBO NUCIFERA GAERTN ON HUMAN NEUROBLASTOMA CELL LINE (SH-SY5Y)

  • KHEMJIRA JARMKOM Department of Thai Traditional Medicine, Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand
  • SURACHAI TECHAOEI Department of Thai Traditional Medicine, Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand
  • NAKUNTWALAI WISIDSRI Department of Thai Traditional Medicine, Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand.
  • WARACHATE KHOBJAI Department of Thai Traditional Medicine, Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand.

Abstract

Objective: Cognitive deterioration occurring in patients with probable Alzheimer’s disease is associated with a progressive loss of cholinergic neurons
and a consequent decline in levels of acetylcholine in the brain. This study aimed to evaluate the acetylcholinesterase (AChE) inhibitory effects and
cytotoxicity in SH-SY5Y cells of different parts of three lotus extracts.
Methods: AChE activity was quantified by spectrophotometry and cytotoxicity by flow 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide
assay in SH-SY5Y cells exposed to extracts.
Results: All of the extracts had inhibitory effects to AChE at p<0.05, but Roseum Plenum stem extract could inhibit AChE more than 30% (p<0.05). The
all of the extracts could be an increase SH-SY5Y cell proliferation, while Album Plenum flower extract could be cytotoxic on SH-SY5Y cells.
Conclusion: The extracts of lotus could be supplemented compound for cognitive deterioration or Alzheimer’s patients.

Keywords: Nelumbo nucifera Gaertn, Acetylcholinesterase activity, Human neuroblastoma cell line

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JARMKOM, K., TECHAOEI, S., WISIDSRI, N., & KHOBJAI, W. (2019). EVALUATION OF ACETYLCHOLINESTERASE ACTIVITY AND CYTOTOXICITY OF DIFFERENT PARTS OF NELUMBO NUCIFERA GAERTN ON HUMAN NEUROBLASTOMA CELL LINE (SH-SY5Y). International Journal of Applied Pharmaceutics, 11(5), 103-105. https://doi.org/10.22159/ijap.2019.v11s5.T0080
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