IN VITRO ANTIMICROBIAL ACTIVITY OF SOME ESSENTIAL OILS AGAINST BACTERIAL PATHOGENS CAUSING SKIN DISEASES IN VAPOR PHASE
Objective: The objective of this study was to evaluate the antimicrobial activity of volatile oils from aromatic plants against pathogenic bacteria.
Methods: Thai aromatic plants such as Pogostemon cablin (Blanco) Benth (Patchouli oil), Cymbopogon nardus Rendle (Citronella grass oil), Pelargonium
roseum (Geranium oil), Syzygium aromaticum (L.) Merrill and Perry (clove oil), Cinnamomum spp.(cinnamon oil), and Cymbopogon citratus (DC.) Stapf.
(lemongrass oil) were selected. Essential oils were obtained by water distillation and were stored at 4°C until use. Five human pathogenic bacteria were
obtained from Thai traditional Medicine College, Rajamangala University of Technology, Staphylococcus epidermidis, Escherichia coli, Staphylococcus
aureus, methicillin-resistant S. aureus (MRSA), and Pseudomonas aeruginosa. The antibacterial activity of volatile oils was determined by disc-diffusion
assay. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each essential oil were determined.
Results: Our study showed that 10% of essential oil from Cinnamomum spp. was the most potential against S. aureus, MRSA, and E. coli when assayed
by disc-diffusion method with inhibition zones ranging from 37.66±0.57 to 45.33±1.15 mm and from 29.33±0.57 to 36.00±1.00 for lemongrass oil
with MIC and MBC of 1.25%.
Conclusion: From this study, it can be concluded that some essential oils have potential antibacterial activity. The present investigation provides
support to the antibacterial properties of essential oils and will be applied to health-care product as aroma antibacterial products.
Moshella S, Hurley HJ, editors. Dermatology. 3rd ed. Philadelphia, PA:
W.B. Saunders; 1992.
2. Peirano G. Multi-resistant enterobacteriaceae new threat to an old prob;
expect review of anti infective therapy. Expert Rev Anti Infect Ther
3. Owlia P, Saderi H, Rasooli I, Sefidkon F. Antimicrobial characteristics
of some herbal oils on Pseudomonas aeruginosa with special reference
to their chemical compositions. Indian J Pharmacol Res 2009;8:107-14.
4. Mubita C, Syakalima M, Chisenga C, Munyeme M, Bwalya M,
Chifumpa G, et al. Antibiograms of faecal Escherichia coli and
enterococci species isolated from pastoralist cattle in the interface areas
of the Kafue basin in Zambia. Vet Arhiv 2008;78:179-85.
5. Chambers HF, Deleo FR. Waves of resistance: Staphylococcus aureus
in the antibiotic era. Nat Rev Microbiol 2009;7:629-41.
6. Karou SD, Nadembega MC, Zeba B, Ilboudo DP,
Ouermi D, Pignatelli S, et al. Évolution de la résistance de
Staphylococcus aureus aux antibiotiques au centre médical Saint
Camille De Ouagadougou. Méd Trop 2010;70:241-4.
7. Hossain MT, Siddique MP, Hossain FM, Zinnah MA, Hossain MM,
Alam MK, et al. Isolation, identification, toxin profile and antibiogram
of Escherichia coli isolated from broilers and layers in Mymensingh
district of Bangladesh. Bangl J Vet Med 2008;6:1-5.
8. Arredondo-García JL, Amábile-Cuevas CF. High resistance prevalence
towards ampicillin, co-trimoxazole and ciprofloxacin, among
uropathogenic Escherichia coli isolates in Mexico city. J Infect Dev
9. Kordali S, Kotan R, Mavi A, Cakir A, Ala A, Yildirim A. Determination
of the chemical composition and antioxidant activity of the essential
oil of Artemisia dracunculus and of the antifungal and antibacterial
activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia
santonicum, and Artemisia spicigera essential oils. J Agric Food Chem
10. World Health Organization. Regional Office for Europe and
International Federation of Red Cross and Red Crescent Societies,
Infections and Infectious Diseases. A Manual for Nurses and
Midwives in the WHO European Region. Geneva: World Health
11. Song A, Wang Y, Liu Y. Study on the chemical constituents of the
essential oil of the leaves of Eucalyptus globulus Labill from China.
Asian J Tradit Med 2009;4:134-40.
12. Bueno J. Models of evaluation of antimicrobial activity of essential
oils in vapour phase: Promising use in healthcare decontamination. Nat
Volatiles Esent Oils 2015;2:16-29.
13. Chen H, Tian T, Miao H, Zhao YY. Traditional uses, fermentation,
phytochemistry and pharmacology of Phellinus linteus: A review.
14. Ács K, Balázs VL, Kocsis B, Bencsik T, Böszörményi A, Horváth G.
Antibacterial activity evaluation of selected essential oils in liquid and
vapor phase on respiratory tract pathogens. BMC Complement Altern
15. Bagamboula CF, Uyttendaele M, Debevere J. Antimicrobial effect of
spices and herbs on Shigella sonnei and Shigella flexneri. J Food Prot
16. Bakkali F, Averbeck S, Averbeck D, Zhiri A, Idaomar M. Cytotoxicity
and gene induction by some essential oils in the yeast Saccharomyces
cerevisiae. Mutat Res 2005;585:1-13.
17. Botelho MA, Nogueira NA, Bastos GM, Fonseca SG, Lemos TL,
Matos FJ, et al. Antimicrobial activity of the essential oil from Lippia
sidoides, carvacrol and thymol against oral pathogens. Braz J Med Biol
18. Castilho PC, Savluchinske-Feio S, Weinhold TS, Gouveia SC.
Evaluation of the antimicrobial and antioxidant activities of essential
oils, extracts and their main components from oregano from Madeira
Island, Portugal. Food Control 2012;23:552-8.
19. Ye CL, Dai DH, Hu WL. Antimicrobial and antioxidant activities of the
essential oil from onion (Allium cepa L.). Food Control 2013;30:48-53.
20. Stefanakis MK, Touloupaskis E, Anastospoulos E, Ghanotakis K,
Katerinopoulos HE, Makridis P. Antibacterial activity of essential oils
from plants of the genus Origanum. Food Control 2013;34:539-46.
21. Ponce AG, Fritz R, Del Valle C, Roura SI. Antimicrobial activity of
essential oils on the native microflora of organic Swiss chard. LWT
Food Sci Technol 2003;36:679-84.
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